Antibody Panel to Vasopressin and Vasopressin Receptors

Introduction

Vasopressin (Arg-8-Vasopressin), the antidiuretic hormone is a cyclic nonapeptide invovled in the homeostasis of body fluid osmolality, blood volume, vascular tone, and blood pressure.

Specific actions of Vasopressin include inhibition of diuresis, contraction of smooth muscle, stimulation of liver glycogenesis, and modulation of ACTH release from pituitary. Vasopressin belongs to the family of vasocative and mitogenic peptide invovled in normal and pathological cell growth and differentiation.

Vasopressin exerts its action through binding to specific membrane receptors coupled to distinct second messengers. There are 3 types of Vasopressin receptors: V1a, V1b, and V2 subtypes. The V2 receptor stimulates adenyl cyclase and protein Kinase A, V1 activate phospholipase A2, C, and D, resulting into production of IP3 and DAG, the mobilization of intracellular calcium, the influx of extracellular calcium, the activation of protein Kinase C, and protein phosphorylation. The V1a receptors mediate vasoconstriction and hepatic gluconeogenesis platelet aggregation, coagulation factor release.

The V1b modulates ACTH secretion from the anterior pituitary and the V2 mediates anti-diuretic effects. V1b receptors are located in anterior pituitary. V2 renal receptors are present in medullary portion of the kidney where they control free water and urea adsorption by stimulating adenyl cyclase.

Vasopressin receptors are members of the G-protein coupled receptors with putative 7 transmembrane domains. The N-terminus and C-terminus are predicted to be extracellular and cytoplasmic, respectively.

Antibody Tools for the Detection of Vasopressin and its Receptors

Acris Antibodies offers an antibody panel for detection of Vasopressin and the V1a, V1b and V2 receptors useful for immunohistochemistry, ELISA and Western blotting techniques.

Fig. 1: Staining of rat brain using anti Vasopressin antibody cat. no. 20069

Fig. 2: Staining of human kidney using anti V2 Receptor antibody cat. no. SP4535P

Fig. 3: Staining of human pituary using anti V1b Receptor antibody cat. no. SP4534P (red), brown double stain is ACTH.

Available Antibody Panel to Vasopressin / Receptors

Vasopressin (Arginine Vasopressin, Antidiuretic Hormone, AVP), Vasopressin V1a Receptor (AVPR1A), Vasopressin V1b Receptor (AVPR1B ), Vasopressin V2 Receptor (AVPR2, ADHR) –antibody

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