Antibodies to Insulin Receptor (CD220)

Introduction

The insulin receptor is a transmembrane protein which consists of 4 subunits (2 alpha 2 beta) and exhibits receptor tyrosine kinase activity (RTK).

RTKs are single-pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma-phosphate of ATP to tyrosine residues in protein substrates.

RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism.

Included in this large protein family are the insulin receptor (IR, INSR) and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins.

Antibody Tools for the Detection of CD220

Acris Antibodies offers a panel of antibodies for the detection of Insulin Receptor (alpha / beta).

These antibodies can be used for immunohistochemistry, Western blotting or other immunological methods.

Fig.1: Formalin-fixed, paraffin-embedded human placenta stained with anti-Insulin Receptor antibody AM00362PU-N using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of trophoblasts.

Fig.2: Paraffin embedded pancreas: Islet of Langerhans stained with anti-Insulin Receptor antibody AP00470PU-N

Available Antibody Panel to Insulin Receptor

Insulin Receptor (CD220, IR, INSR) antibody, Insulin Receptor alpha and Insulin Receptor beta antibody

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