Antibodies to SET1, SET7 and SET7/9

Introduction: Eukaryotic DNA is assembled with histone proteins to form nucleosomes, which are the basic repeating unit of chromatin. The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception.

Human SET1 (hSET-1) is a histone methyltransferase that may play a role in chromatin modification and meiotic differentiation.

SET7 Methyltransferase methylates lysine 4 (Lys 4) of histone H3. Methylation occurs at the e amino group of lysine residues. Di- and tri- methylation of histone H3 Lys 4 is a hallmark of transcriptionally active chromatin and is globally distributed.

Set7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.

Antibody Tools for the Detection of Methyltransferases

Acris Antibodies offers antibodies to SET1, SET7 and SET7/9 which can be used in Western blot applications and / or ELISA.

Recombinant Human Set7/9 histone methyltransferase is also available.

Fig.1: Detection of Human hSET1 by Western Blot. Samples: Whole cell lysate (WCL; 100 or 200 mcg) and nuclear extract (NE; 100 and 200 mcg) from HEK293T cells. Antibody: NB 100-557 used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of 1 minute.

Fig.2: SET7 polyclonal antibody catalog no. H00080854-A01: Western Blot detection against Immunogen (38.21 KDa).

Fig.3: Western blot analysis for Set7/9 using SP7167 at 1:500 on 20 µg of HeLa whole cell lysate.

Fig.4: Recombinant human Set7/9 histone methyltransferase, E.coli, purity = 95% by SDS-PAGE

Available Antibodies to H3 Lys4 Histone Methyltransferases

hSET1 (hSET-1, SET-1) antibody, SET7 antibody, SET7/9 antibody

Literature:

Scott D. Briggs, Mary Bryk, Brian D. Strahl, Wang L. Cheung, Judith K. Davie, Sharon Y.R. Dent, Fred Winston, and C. David Allis. Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae Research paper Genes & Development Vol. 15, No. 24, pp. 3286-3295, December 15, 2001

Wang,H., Cao,R., Xia,L., Erdjument-Bromage,H., Borchers,C., et al. Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. Mol. Cell 8 (6), 1207-1217 (2001).

Nishioka,K., Chuikov,S., Sarma,K., Erdjument-Bromage,H., Allis,C.D., Tempst,P. and Reinberg,D. Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation. Genes Dev. 16 (4), 479-489 (2002).

Wysocka,J., Myers,M.P., Laherty,C.D., Eisenman,R.N. and Herr,W. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. Genes Dev. 17 (7), 896-911 (2003).

Kwon, T., Chang, JH., Kwak, E., Lee, CW., Joachimiak, A., Kim, YC., Lee, J., Cho. Y. Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9 AdoMet. EMBO J. 22(2):292-303 (2003).