Antibodies to Mouse Macrophages

Introduction: In this short article we present the most important products for detection of mouse macrophages in different tissues.

( For antibodies to human and rat macrophages see Focus on 10, 27 and 46 ).

F4/80 Antigen

Clones BM8 and CI:A3-1 recognizes the F4/80 antigen, a 120-160kD glycoprotein containing 7 EGF-like domains at the N-terminus, an RGD (Arg-Gly-Asp) integrin binding motif, and significant homology to the transmembrane 7 (Tm7) hormone receptor family at the C-terminus. The molecular structure suggests multiple ligands. Possibly, F4/80 is involved in macrophage adhesion within certain tissues, combined with receptor signaling following peptide-ligand interaction. The antigen is expressed by most macrophages and macrophage precursors beyond M-CFC, and increases upon maturation. F4/80 is widely used for the detection of tissue macrophages. Activated macrophages, and eosinophils express low levels. Schaller et a. (2002) showed that BM8 antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to ß-cell destruction and diabetes in a mouse diabetes model.

The MOMA Clones

The clone MOMA-1 and MOMA-2 are widely used mouse macrophage subpopulation markers. MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterised by a high level of non-specific esterase expression. The staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. The marker is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by ER-TR9. In addition , MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells. MOMA-2 is a useful marker for the broad detection of monocytes and macrophages in all mouse strains. In combination with the anti F4/80 marker BM8 or Cl:A3-1 it allows a precise characterisation of tissue fixed macrophages in various organs. The antibody stains a mature macrophage subset, monocytes and a few precursors in bone marrow. Dendritic cells show low to intermediate expression. MOMA-2 is predominantly expressed in the cytoplasm, but is also present on the cell surface. The major drawback of both clones is that they cannot be used on paraffin sections like BM8 or Cl.A3-1.

Antibody to Macrophage Precursor Cells (Ly-6C) Clone ER-MP20

ER-MP20 antibody is useful for the detection of macrophage precursor cells in the mid-stage development stage (Ly-6C, late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ER-MP20 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti F4/80) is recommended. ER-MP20 antibody also detects a wide range of endothelial cells.

Available antibody panel to Mouse Macrophages

Antibody to: F4/80 antigen, Metallophilic Macrophages, Macrophages/Monocytes, Ly-6C (Macrophage precursor cells)

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