Antibodies to Loading Controls

Western blot analysis some times is used for (semi-) quantitative detection of target proteins, especially concerning experimental procedures which lead to altered expression levels of the target protein. This requires a method to standardize not only the amount of total protein loaded per lane, but also to standardize the amount of detectable marker proteins, which ideally should not show an altered expression level during the course of the experiment.

For this purpose so called ‘house keeping’ proteins are selected as controls. These proteins are of fundamental importance in maintaining basic cellular functions. This group is composed of enzymes involved in central biochemical pathways (such as GAPDH) or highly conserved structural proteins (such as Actin and Tubulin).

Antibodies

Acris Antibodies offers offers a wide range of antibodies which are suitable for use as loading controls. The table below shows a selection of our currently available antibodies.

All loading control antibodies: Actin loading control antibody, GAPDH loading control antibody, Profilin loading control antibody,, TBP loading control antibody, Tubulin loading control antibody, VDAC loading control antibody

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