Antibodies to Gastrin-releasing Peptide (Bombesin) and its Receptors (BRS-3 ,GRP-R and Neuromedin B-Receptor)

Introduction

Bombesin (BN) is a tetradecapeptide (Pyro-ENR LGN QWA VGH LM-amide) that was originally isolated from frog skin.

Several BN-related peptides have been isolated from amphibians and classified into three subfamilies: Bombesin, ranatensin, and phyllolitorin based upon the last three C-terminal residues. Two BN-like peptides have been identified in mammals: NMB (neuromedin B) in the ranatensin family and GRP (gastrin-releasing peptide) in the BN-family. BN-family of G-protein coupled receptors include at least four receptor subtypes: The GRP-preferring receptors (GRP-R or bb2), the NMB-preferring receptors (NMB-R or bb1) and Bombesin receptor subtype 3 (BRS-3 or bb4). These receptors share approx. 50% amino acid homology and bind bombesin. However, BRS-3 has much lower affinity for BN than GRP-R and NMB-R. Mammalian BN-like peptides are widely distributed in the brain and gastrointestinal tract, where they modulate smooth-muscle contraction, exocrine and endocrine activities, metabolism and behavior. Most recently, BRS-3 deficient mice have been shown to develop mild obesity, associated with hypertension, and impairment of glucose metabolism, reduced metabolic rate, increased feeding efficiency and subsequent hyperphagia. Human BRS-3 gene (chromosome x) encodes a 399 aa protein with seven transmembrane domains. The NH2 and COOH-termini are predicted to be extracellular and cytoplasmic, respectively.

Antibody Tools for the Detection of Bombesin and Bombesin Receptors

Acris Antibodies offers a range of antibodies for detection of Bombesin and its receptors (GRP-R, BRS-3 and Neuromedin B Receptor). Products can be used for immunological applications like immunohistochemistry (see pictures below).

Fig. 1: Staining of rat dorsal horn using anti Bombesin antibody Catalog No. 20073

Fig. 2: Staining of Pancreas (Islet of Langerhans) using anti GRP-R antibody Catalog No. SP4337P

Fig. 3: Staining of human Placenta using antibody to BRS-3 Catalog No. SP4074P (N-term)

Fig. 4: Staining of human Placenta using antibody to BRS-3 Catalog SP4075P(C-term)

Fig. 5: Staining of brain (olfactory bulb) using anti Neuromedin B Receptor antibody Catalog No. SP4414P

Fig. 6: Staining of brain (pituitary) using anti Neuromedin B Receptor antibody Catalog No. SP4598P

Available Antibody Panel to Gastrin-releasing Peptide (Bombesin) and its Receptors

Anti- Bombesin (Gastrin-releasing Peptide, GRP), Neuromedin B receptor (NMBR), Gastrin-Releasing Peptide Receptor (GRPR), Bombesin Receptor Subtype 3 (BRS3) antibody

27 related products - click here to filter product result list