Antibodies to Flotillin 1 & 2

Introduction

Flotillins are integral membrane proteins that have been shown to be present in several subcellular components, including caveolae (invaginated plasma membrane microdomains), lipid rafts (sphingolipid and cholesterol-rich, detergent-resistant plasma membrane microdomains), and the Golgi apparatus. The molecular function of flotillins remains uncertain. They are probably involved in organising the structure of caveolae and lipid rafts, and other detergent resistant membrane domains. They may also be involved in signal transduction. Flotillins have been shown to accumulate in brain cells with the development of Alzheimer's pathology . Also included in this family are Reggie proteins, which are expressed in non-caveolar neuronal plasma membrane domains.

Antibody Panel to Flotillin

Acris Antibodies offers two polyclonal antibodies to Flotillin 1 and Flotillin 2, respectively. Each Flotillin-antibody is suitable for use in Western blotting and ELISA application (see pictures and table below.)

Flotillin 1: Antibody SP7253P (0.3 µg/ml) staining (0.3 µ g/ml) of H460 lysate (RIPA buffer, 35 µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Flotillin 2: Antibody SP7321P (0.3 µ g/ml) staining of human lung lysate (35 µ g protein in RIPA buffer). Primary incubation was 1 hour. Detected by western blot using chemiluminescence

References:

1. Ref. 1:

2. Rajendran L, Masilamani M, Solomon S, Tikkanen R, Stuermer CA, Plattner H, Illges H. Asymmetric localization of flotillins/reggies in preassembled platforms confers inherent polarity to hematopoietic cells. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8241-6. PMID: 12826615

3. Ref. 2:

4. Kokubo H, Lemere CA, Yamaguchi H. Localization of flotillins in human brain and their accumulation with the progression of Alzheimer's disease pathology. Neurosci Lett 2000;290:93-96.

5. Ref. 3:

6. Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem 1997;272:13793-13802.

7. Ref. 4:

8. Pfam database of protein families and HMMs; Washington University in St. Louis (August 2005).