Antibodies to Dopamine, Dopaminehydroxylase (DBH), Dopamine-transporter and its Receptors

Introduction

Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intra¬¬cellular ion transport. For example, degeneration of certain dopamine-producing brain cells results in parkinsonism.

A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short D2S, are encoded by splice variants of a single gene and differ only by the presence of an additional 29 aa in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative trans¬membrane domains.

Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system.

The dopamine transporter (DAT) is a phosphoprotein whose activity and phosphorylation state are acutely regulated by both protein kinase C (PKC) and substrate transport. DAT is a major site of action for psycho¬¬¬¬¬¬stimulant and therapeutic drugs that either block transport or are transported substrates.

Antibody Panel to Dopamine, DBH, Dopamine-transporter and Its Receptors

Acris Antibodies offers a range of monoclonal and polyclonal antibodies to to Dopamine and its beta¬-hydroxylase, transporter and receptors suitable for Immunohistochemical applications and / or Western blotting.

Fig. 1: Transfected cells expressing Dopamine receptor D1 immunostained with antibody SP4112P.

Fig. 2: Immunohistochemical staining of Dopamine receptor D2 in human brain sections (showing neurons and glia) with antibody SP4562P.

Antibody Panel to Dopamine, DBH, Dopamine-transporter and Its Receptors

Dopamine (D1-D5), DBH, Dopamine-transporter (DATs) and its Receptors antibody.

Literature

Cervinski MA, Foster JD, Vaughan RA. Psychoactive substrates stimulate dopamine transporter phosphorylation and down regulation by cocaine sensitive and protein kinase C dependent mechanisms. 2005 Dec 9;280(49):40442-9.

Wilcox KM, Kimmel HL, Lindsey KP, Votaw JR, Goodman MM, Howell LL. In vivo comparison of the reinforcing and dopamine transporter effects of local anesthetics in rhesus monkeys. Synapse. 2005 Dec 15;58(4):220-8.

Gorentla BK, Vaughan RA. Differential effects of dopamine and psychoactive drugs on dopamine transporter phosphorylation and regulation. Neuropharmacology. 2005 Nov;49(6):759-68.

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