Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD)

Introduction

The DNA fragmentation factor (DFF) is composed of two subunits, the 40-kDa caspase-3-activated nuclease (DFF40/CAD) and its 45-kDa inhibitor (DFF45/ICAD).

Apoptosis is induced through a number of cell death receptors and their ligands like TNF and TNF receptor. The signaling cascade is carried further by adaptor proteins containing death domains and by caspase family of proteins. The caspase-activated DNase (CAD) is the primary nuclease responsible for oligonucleosomal DNA fragmentation during apoptosis. DFF45/ICAD acts as a chaperon for appropriate folding of DFF40 and it also acts as inhibitor of DFF40. Caspase 3 upon activation cleaves DFF to release DFF40/CAD, which oligomerizes into a large active complex and causes the degradation of DNA in nuclei. Hence cleavage of DFF45 is an important step in apoptosis.

Antibody Panel to DFF (CAD and ICAD)

Acris Antibodies offers a range of monoclonal and polyclonal antibodies to DFF40 and DFF45 suitable for Western blotting, Immunohistochemistry and / or other immunological detection methods.

Fig. 1: Anti-DFF40 (CAD) Western blot analysis of 30 µg of HeLa cell lysate with antibody SM7025P at a dilution of 2 µg/ml. A 40 kDa band is observed.

Fig. 2: Anti-DFF45 (ICAD) Western blot analysis of 50 µg of total cell lysate from HeLa cells with antibody SP7059P at 1:2000 dilution. A 45 kDa band is observed.

Fig. 3: Anti-DFF45 (ICAD) Western blot of antibody SP5430P in HeLa cell lysate.

Fig. 4: Anti-DFF45 (ICAD) Western blot of antibody SP5431P in HeLa cell lysate.

Antibody Panel to DFF

DNA Fragmentation Factor DFF40 (CAD) and DFF45 (ICAD) antibody.

Literature

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Lechardeu D, Dougaparsad S, Nemes C, Lukas GL. Oligomerization state of the DNA fragmentation factor (DFF) in normal and apoptotic cells. J Biol Chem. 2005 Dec 2; 280(48): 40216-25.

Widlak P, Garrard WT. Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD and endonuclease G. J Cell Biochem. 2005 Apr 15; 94(6): 1078-87

Korn C, Scholz SR, Gimadutdinow O, Lurz R, Pingoud A, Meiss G. Interaction of DNA Fragmentation Factor (DFF) with DNA reveals an unprecedented mechanism for Nuclease inhibition and suggests that DFF can be activated in a DNA-bound state. J Biol Chem. 2005 Feb 18; 280 (7): 6005-6015

Eckhart L, Fischer H, Tschachler E. Phylogenomics of caspase-activated DNA fragmentation factor. Biochem Biophys Res Commun. 2007 Apr 27;356(1):293-9.

Inohara N, Koseki T, Chen S, Benedict MA, Núñez G. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J Biol Chem. 1999 Jan 1;274(1):270-4.

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