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Western blot analysis of ARK in HCT-116 whole cell lysate using antibody SP7343P at 4 ug/ml.

 

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SP7343P  Aurora-A/B / STK12/15 / ARK1/2 antibody

see related secondary antibodies
0.1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Aurora-A/B / STK12/15 / ARK1/2 -

Synonyms

STK6/5, AURKA/B, ,

Product review
Please read our product review about Aurora-A/B / STK12/15 / ARK1/2: Antibody Panel for the Detection of Aurora Kinase Family.

Product Description

Rabbit anti Aurora-A/B / STK12/15 / ARK1/2 -, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )

Properties

ApplicationsWestern blot / Immunoblot ( WB )
ReactivityHuman ( Hu ), Mouse ( Ms ), Rat ( Rt )
PresentationPurified
HostRabbit
Catalog NumberSP7343P
Swiss Prot. No.Q96GD4
Quantity0.1 mg
PriceUS$ 405
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SP7343P.pdf

Datasheet Extract

Polyclonal Antibody to Human ARK1/2
Alternate namesSTK12/15, Aurora A/B
Concentration0.5 mg/ml
BackgroundThe Aurora gene, a serine-threonine kinase, orginally discovered in Drosophila, has been found to be responsible for a chromosomal segregation defect. The mammalian homologues of Drosophila Aurora have been termed ARK1 (Aurora-related kinase 1) and ARK2.
ImmunogenThis antibody was developed against KLH-conjugated synthetic peptides corresponding to amino acids 2-16 and 157-170 of human ARK.
Swiss Num.: Q96GD4
Remarks: These sequences are 100% homologous in ARK1 and ARK2.
These sequences are over 80% homologous in mouse and rat.
Format
State: Liqud IgG fraction
Purification: Protein G affinity chromatography
BufferSystem: PBS containing 0.2% gelatin and 0.05% sodium azide as preservative
ApplicationsWestern blot: 2.0 - 4.0 µg/ml.
In HCT-116, a 48 kDa band is observed.
Recommended positive control: HCT-116.
SpecificityThis antibody reacts with ARK1 and ARK2.
Species: Human (predicted reactivity: mouse and rat).
StorageStore the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Shindo,M., Nakano,H., Kuroyanagi,H., Shirasawa,T., Mihara,M., Gilbert,D.J., Jenkins,N.A., Copeland,N.G., Yagita,H. and Okumura,K. cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2. Biochem. Biophys. Res. Commun. 244 (1), 285-292 (1998).
PicturesWestern blot analysis of ARK in HCT-116 whole cell lysate using antibody SP7343P at 4 ug/ml.


12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SP7343P Aurora-A/B / STK12/15 / ARK1/2'.