SP6388P GFP antibody
see related secondary antibodiessee all 64 GFP products
50 µg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti GFP -
Synonyms
Green Fluorescent Protein
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Rabbit anti GFP -, Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP6388P |
| Swiss Prot. No. | P42212 |
| Quantity | 50 µg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP6388P.pdf |
Datasheet Extract
| Polyclonal Antibody to GFP Tag | |
| Concentration | 0.2 mg/ml |
| Background | Green fluorescent protein (GFP) is a 27 kDa protein which was originally cloned from Jellyfish cnidarians, Aequorea victoria. This exceptional protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509 nm) without any requirement of exogenous substrates and cofactors (1). These unique qualities of its intrinsic fluorescence make GFP become an invaluable tool in cell biology research such as monitoring gene expression and protein localization of GFP-tagged proteins in vivo. Other applications of GFP also include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maximum when compared to the wild type GFP, making them useful for FACS, fluorescence microscopy, and double-labeling applications (2, 3). |
| Immunogen | Highly purified green-fluorescent protein (GFP) isolated directly from Aequorea victoria Swiss Num.: P42212 |
| Format | State: Liquid IgG fraction Purification: Antigen affinity chromatography. BufferSystem: 1 x PBS, pH 7.4, containing 0.05 % sodium azide |
| Applications | ELISA: 1:5,000-1:10,000.
Western blot: 0.25-1.0 µg/ml. Immunoprecipitation: 2.0-5.0 µg/extract from 10e7 cells. Immunohistochemistry: 2.0-5.0 µg/ml. |
| Specificity | This antibody recognizes all variants of recombinant Aequorea GFP. |
| Storage | Store at 2-8°C for up to one year from despatch. |
| References | 1. Chalfie M. et al, Science 263: 802-805, 1994.
2. Cormack B.P. et al, Gene 173: 33-38, 1996. 3. Rizzuto R. et al, Curr.Biol. 6: 183-188, 1996. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 € | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 € | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 € | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 € | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 € | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 € | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'SP6388P GFP'.
