SP6372P I Kappa B alpha (IKBa) non pSer32/36 antibody
see related secondary antibodies
50 µg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti I Kappa B alpha (IKBa) non pSer32/36 -
Synonyms
IkappaB alpha
Product Description
Rabbit anti I Kappa B alpha (IKBa) non pSer32/36 -, Presentation: Aff - Purified. Product is tested for Immunoprecipitation ( IP ), Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP6372P |
| Quantity | 50 µg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP6372P.pdf |
Datasheet Extract
| Polyclonal Antibody to I Kappa B alpha (IKBa) non pSer32/36 | |
| Concentration | 0.2 mg/ml |
| Background | NF-κB is a family of transcription factors, including p50, p52, p65, RelB, and C-Rel (1). It regulates the expression of a number of proteins involved in the regulation of cell survival and immune responses (2). The biological activity of NF-κB is tightly controlled by its inhibitor protein IκB, which binds to and sequesters NF-κB in the cytoplasm. There are three isoforms of the IκB: IκBα, -β and -ε. IκBα, the well-characterized member of the IκB family, contains both nuclear localization sequence and nuclear export signal that render to IκBα the activity to rapidly shuttle between the nucleus and the cytoplasm. The IκBα protein binds to NF-κB dimmers, masking their nuclear localization sequences and causing their cytoplasmic retention. After NF-κB positive stimuli, IκB kinase complex phosphorylates IκBs in two conserved serines (Ser-32 and Ser-36 in IκBα), leading to ubiquitination and subsequent proteasomal degradation (1). |
| Immunogen | Synthetic peptide corresponding to human IκBα around serines 32 and 36 AA Sequence: RHDSGLD |
| Format | State: Liquid Ig fraction Purification: Epitope affinity chromatography BufferSystem: 1x PBS (pH 7.4) containing 0.05 % sodium azide |
| Applications | ELISA: 0.1-1.0 µg/ml.
Western blot: 0.5-2.0 µg/ml. Immunoprecipitaion (recommended): 3.0-5.0 µg/extract from 10e7 cells. |
| Specificity | This antibody is specific for human IκBα. It was evaluated by ELISA, dot blot and Western blot. It only recognized the human IκB, not other phosphorylated or non-related peptides. By immunoblot assay, an immunoreactive band at ~40 kDa from Hela, 3T3, THP-1 whole cell lysate was detected. This immunoreactive band is abolished by the depletion of the immunizing peptide. Species: Human, Mouse, Rat. |
| Storage | Store the antibody at 2 - 8 °C or (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Karin, M et al (2000) Annu. Rev. Immunol. 18: 621-663. 2. Karin, M. et al. (2001) Nat. Immunol. 3: 221-227. |
| Pictures | Western blot analysis: Whole cell lysate, derived from treated HeLa, was probed by anti- IκB (SP6372P, Lane 1) or by pre-absorbed antibody with immunizing peptide (Lane 2) |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 € | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 € | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 € | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 € | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 € | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 € | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'SP6372P I Kappa B alpha (IKBa) non pSer32/36'.
