SP6201P Estrogen Receptor alpha / ESR1 (pSer167) antibody
see related secondary antibodiessee all 119 Estrogen Receptor alpha / ESR1 products
50 µg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Estrogen Receptor alpha / ESR1
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Rabbit anti Estrogen Receptor alpha / ESR1 , Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP6201P |
| Swiss Prot. No. | P03372 |
| Quantity | 50 µg |
| Price | US$ 375 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP6201P.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | The estrogen receptor α (ERα), a transcription factor that controls the expression of a number of genes involved in cellular differentiation and proliferation in a wide variety of tissues (1, 2), is regulated by ligand binding and phosphorylation. ER contains at least two transcriptionally active domains: constitutively active F-1 at the ER N-terminus of the protein and ligand-dependent AF-2 at the ER C-terminus (3, 4). Although ligand binding is considered essential for the full activation of ER, it has long been recognized that the receptor is subject to post-translational alterations, such as phosphorylation, which also regulated its activity (5). ER phosphorylation patterns appear to be cell type-specific. Serine 167 has been shown to be phosphorylated by p90rsk1 in vitro and to regulate ER AF-1-dependent transcriptional activation in vivo (6). |
| Immunogen | Synthetic peptide corresponding to human ER at the phosphorylated site of Serine 167 Swiss Num.: P03372 |
| Format | State: Liquid Ig fraction Purification: Epitope affinity chromatography BufferSystem: 1x PBS (pH 7.4) with 0.05 % sodium azide |
| Applications | ELISA (0.1 - 1.0 µg/ml).
Western blotting (0.5 - 2 µg/ml, detects a band of ~60 kDa). Immunoprecipitation (3.0 - 5.0 µg/extract from 10e7 cells). |
| Specificity | This antibody is specific for ER-Alpha (pSer167).
It was evaluated for specificity with a dot blot assay using synthetic ER peptides. Species: Human, Mouse, Rat. |
| Storage | Store at 2 - 8 °C.
Shelf life: one year from despatch. |
| References | 1. Blobel GA and Orkin SH (1996) Mol. Cell. Biol. 16: 1687-1694
2. Gaub MP et al (1990) Cell 63: 1267-1276. 3. Kraus WL, e al (1995) Proc. Natl. Acad. Sci. U.S.A. 92: 12314-12318. 4. McInerney Em et al (1996) Proc. Natl. Acad. Sci. U.S.A. 93: 10069-10073. 5. Rofatsky I et al (1999) J. Biol. Chem. 274 (32): 22296-22302. 6. Joel PB et al (1998) Mol. Cell. Biol. 18: 1978-1984. |
| Pictures | Whole cell lysate from stimulated MCF-7 was probed with anti-ER-alpha (pSer167) (Lane 1) or by pre-absorpted antibody with immunizing peptide (lane 2). |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP6201P Estrogen Receptor alpha / ESR1 '.
