SP5327P Dnmt1 antibody
see related secondary antibodiessee all 50 Dnmt1 products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Dnmt1 -
Synonyms
M.Hsal, MCMT, AIM, CXXC9, DNMT, , DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, CXXC-type zinc finger protein 9
Product review
Please read our product review about Dnmt1 : Antibodies to DNA methyltransferases (Dnmt Family).
Product Description
Rabbit anti Dnmt1 -, Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP5327P |
| Swiss Prot. No. | P26358 |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5327P.pdf |
Datasheet Extract
| Polyclonal Antibody to Human //DNA Methyltransferase 1 (Dnmt1) | |
| Concentration | 1.0 mg/ml |
| Background | DNA methyltransferases (Dnmts) methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions. There exist three isoforms of Dnmts, Dnmt1, 2 and 3. Dnmt1 is believed to perform most of the maintenance and de novo methylation activities that occur in somatic cells of mammals. Studies have shown that Dnmt1 can establish repression of transcription complexes consisting of Dnmt1, histone deacetylase 2 (HDAC2), and DNA methyltransferase 1 associated protein (DMAP1). Therefore, in addition to DNA methylation maintenance, Dnmt1 is thought to directly target transcriptionally repressive chromatin to the genome during S-phase DNA replication. |
| Immunogen | Synthetic Peptide: A(171) K G P A K R K P Q E E S E(185) corresponding to amino acid residues 171-185 from human Dnmt1 protein. Swiss Num.: P26358 Remarks: SP5327P immunizing peptide (SP5327CP) is available for use in Neutralization and Control experiments. |
| Format | State: Liquid purified IgG fraction. Purification: Epitope affinity chromatography. BufferSystem: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative. |
| Applications | Western Blot: 0.5-1.0 µg/ml, detects an ~180 kDa protein representing Dnmt1 from HeLa cell nuclear extract.
A doublet between 40 and 45 kDa is also seen on Western blots using this antibody. |
| Specificity | SP5327P detects DNA methyltransferase 1 (Dmnt1) from HeLa cell nuclear extracts. Species: Human. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Cell Growth Differ., 11(10):551-9, 2000.
2. J. Biol. Chem., 276(10):6930-6, 2001. 3. J. Cell Sci., 113 (Pt 22):3887-8, 2000. |
| Pictures | Figure 1. Western blot of Dnmt1 on HeLa cell nuclear extract using SP5327P antibody. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5327P Dnmt1 '.