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<b>Figure 1.</b> Western blot of SCAMP 5 on rat brain extract using SP5254P.

 

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SP5254P  SCAMP5 antibody

see related secondary antibodies
see all 4 SCAMP5 products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti SCAMP5

Product Description

Rabbit anti SCAMP5 , Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )

Properties

ApplicationsWestern blot / Immunoblot ( WB )
ReactivityRat ( Rt )
PresentationAff - Purified
HostRabbit
Catalog NumberSP5254P
Quantity0.1 mg
PriceUS$ 455
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SP5254P.pdf

Datasheet Extract

Alternate namesSecretory Carrier Membrane Protein 5
Concentration1.0 mg/ml IgG
BackgroundSecretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. SCAMPs are composed of four central transmembrane regions and a cytoplasmic tail. Of the five known SCAMPs, SCAMPs 1-3 contain cytoplasmic N-terminal regions with NPF repeats. NPF repeats are found to interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMPs 4 and 5 lack the N-terminal NPF repeats. SCAMPs 1-4 are all ubiquitously coexpressed while SCAMP 5 is only detectable in the brain. Studies show that SCAMP 5 is expressed late in development which is coincident with expansion of mature synapses.
ImmunogenSynthetic Peptide: C N(217) Q P Q T Q Y S A T P N Y T Y S N(233) corresponding to amino acid residues 217-233 from rat SCAMP 5.
Remarks: This sequence is 94% conserved in mouse.
The immunizing peptide (SP5254CP) is available for use in Neutralization and Control experiments.
Format
State: Liquid purified IgG fraction.
Purification: Epitope affinity chromatography.
BufferSystem: PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative.
ApplicationsSuitable for use in Western Blot (2 µg/ml, detects an ~25 kDa protein representing SCAMP 5 from rat brain extract).
SpecificityThis antibody detects secretory carrier membrane protein 5 (SCAMP 5) from rat brain extract.
StorageStore the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Cell Tissue Res., 296:499-510, 1999.
2. J. Cell Sci., 110:2099-2107, 1997.
3. Mol. Biol. Cell, 9(7):1661-1674, 1998.
PicturesFigure 1. Western blot of SCAMP 5 on rat brain extract using SP5254P.

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SP5254P SCAMP5'.