SP5252P Visual Arrestin antibody
see related secondary antibodiessee all 6 Visual Arrestin products
0.2 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Visual Arrestin -
Synonyms
,
Product review
Please read our product review about Visual Arrestin: Antibodies to Arrestin/beta-Arrestin Protein Family.
Product Description
Rabbit anti Visual Arrestin -, Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP5252P |
| Swiss Prot. No. | Q963B5 |
| Quantity | 0.2 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5252P.pdf |
Datasheet Extract
| Polyclonal Antibody to Bovine Visual Arrestin | |
| Concentration | 1.0 mg/ml |
| Background | Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.
Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin. |
| Immunogen | Synthetic Peptide: C E(347) V A T E V P F R L M H P Q P E D(363) corresponding to amino acid residues 2-18 from rat visual arrestin. Swiss Num.: Q963B5 Remarks: This peptide (SP5252CP) is available for use in Neutralization and Control experiments. |
| Format | State: Liquid purified IgG fracion Purification: Epitope affinity chromatography BufferSystem: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative. |
| Applications | Western Blot: 3 µg/ml, detects an ~57 kDa protein representing recombinant bovine visual arrestin. This antibody also detects a lower molecular weight protein which could correspond to degradation product.
Immunofluorescence: 1/1000. |
| Specificity | This antibody detects recombinant bovine visual arrestin.
|
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. J. Biol. Chem., 276(35):32648-56, 2001.
2. Methods Enzymol., 343:600-11, 2002. 3. J. Biol. Chem., 276(35):32648-56, 2001. IOVS 46(1):12-16, 2005. |
| Pictures | Figure 1. Western blot on recombinant bovine visual arrestin using SP5252P. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5252P Visual Arrestin'.