SP5101P KDEL antibody
see related secondary antibodiessee all 12 KDEL products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti KDEL
Product Description
Rabbit anti KDEL , Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Hamster ( Hst ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | SP5101P |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5101P.pdf |
Datasheet Extract
| Background | The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP78) and GRP94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| Immunogen | Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94. |
| Format | State: Lyophilized purified IgG fraction containing 0.05% sodium azide as preservative. Reconstitution: Restore with PBS. |
| Applications | Western Blot: 2 µg/ml, detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ~94 kDa protein representing GRP94 in mouse myeloma cells.
Immunofluorescence: staining of GRP78 in mouse myeloma cells with SP5101P results in diffuse cytoplasmic staining consistent with specific ER localization Immunoprecipitation: SP5101P immunoprecipitates PDI, GRP78, an ~72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ~94 kDa protein representing GRP94 from numerous rodent tissues. |
| Specificity | This antibody detects KDEL from rodent species only, including rat, mouse and hamster.
This antibody does not detect KDEL from human cell lines or COS cells. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Archives of Biochemistry and Biophysics, 296: 129-136, 1992.
2. J. Biol. Chem., 272(7): 4591-4599, 1997. 3. Nature, 355: 33-45, 1992. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5101P KDEL'.
