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Immunohistochemical staining of Breast Carcinoma using anti- EDG5 antibody SP4497P

 

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SP4497P  EDG-5 / S1P2 / S1PR2 antibody

see related secondary antibodies
see all 9 EDG-5 / S1P2 / S1PR2 products
50 µg / no delivery possible
please contact the manufacturer

Quick Overview

Rabbit anti EDG-5 / S1P2 / S1PR2 -

Synonyms

EDG5 H218 AGR16 LPB2 endothelial differentiation, sphingolipid G-protein-coupled receptor 5, Sphingosine 1-phosphate receptor 2, Sphingosine 1-phosphate receptor Edg-5

Product review
Please read our product review about EDG-5 / S1P2 / S1PR2 : Antibodies to Endothelial Differentiation Gene Receptors (EDG-1,-2,-4,-5,-6,-7 and -8).

Product Description

Rabbit anti EDG-5 / S1P2 / S1PR2 -, Presentation: Aff - Purified. Product is tested for Immunofluorescence ( IF ), Paraffin Sections ( P )

Properties

ApplicationsImmunofluorescence ( IF ), Paraffin Sections ( P )
ReactivityHuman ( Hu ), Mouse ( Ms ), Rat ( Rt )
PresentationAff - Purified
HostRabbit
Catalog NumberSP4497P
Swiss Prot. No.O95136
Quantity50 µg
Priceno delivery possible
DeliveryNot USA/Canada
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SP4497P.pdf

Datasheet Extract

Polyclonal Antibody to Sphingolipid Receptor Edg5/S1P2
Alternate namesGPCR13, H218, LPB2, S1PR2, AGR16, endothelial differentiation sphingolipid g-protein-coupled receptor 5
Concentration1.0 mg/ml
BackgroundEDG5, a Lysophospholipid/Lysosphingolipid Receptor, mediates sphingosine-1-phosphate-induced cell proliferation, survival, and transcriptional activation.
EDG5 has been reported in aorta, heart, and skeletal muscle. In rats, it is more highly expressed early during development than postnatally.
Ligand: Sphingosine-1-phosphate
ImmunogenSynthetic peptide - KLH conjugated
Epitope: 2nd cytoplasmic domain of human
Genename: EDG5
Swiss Num.: O95136
Remarks: The immunizing peptide shows 88 % identity (94 % similarity) with mouse and
94 % identity (94 % similarity) with rat.
Format
State: Liquid Ig fraction
Purification: Peptide immunogen affinity column
BufferSystem: PBS, pH 7.7, containing 0.01% sodium azide
ApplicationsImmunocytochemistry.
Immunohistochemistry with formalin fixed paraffin embedded tissues: 11 µg/ml
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
SpecificityReacts with Sphingolipid Receptor Edg5/S1P2
(Family: GPCR, Subfamily: Lysophospholipid / Lysosphingolipid)

Species: Human.
Add. InformationThis product was originally produced by MBL International.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
PicturesImmunohistochemical staining of Breast Carcinoma using anti- EDG5 antibody SP4497P

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SP4497P EDG-5 / S1P2 / S1PR2 '.