SP2162P Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
see related secondary antibodiessee all 49 Glyceraldehyde-3-Phosphate Dehydrogenase products
0.1 mg / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) -
Synonyms
Loading Control, GAPD, G3P, ,
Product review
Please read our product review about Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH): Antibodies to Loading Controls.
Product Description
Rabbit anti Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) -, Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP2162P |
| Swiss Prot. No. | P04406 |
| Quantity | 0.1 mg |
| Price | US$ 385 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP2162P.pdf |
Datasheet Extract
| Polyclonal Antibody to GAPDH C-Terminal | |
| Concentration | 1.0 mg/ml |
| Background | GAPDH is a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes.
GAPDH is a homotetramer molecule consisting of four 36 kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate. The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to Ã?-amyloid precursor protein (Alzheimer's disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdon's disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases (1). |
| Immunogen | A 16 amino acid peptide located near human GAPDH carboxy-terminus Swiss Num.: P04406 |
| Format | State: Liquid Ig fraction Purification: Affinity chromatography BufferSystem: Phosphate buffered saline, 0.02 % Sodium Azide (NaN3) |
| Applications | Western blot (0.5 - 1 µg/ml, detects a band of approximately 37 kDa in HeLa cell lysates). |
| Specificity | This antibody recognises an epitope within the C-terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots, undiluted) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch. |
| References | 1. Mazzola, J.L. and Sirover, M.A (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimerâ??s disease and in Huntingtonâ??s disease fibroblasts. J. Neurochem. 76: 442-449.
2. Sirover, M.A. (2005) New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell Biochem. 95: 45-52. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||