SM7149P Progesterone Receptor (pSer190) antibody
see related secondary antibodiessee all 77 Progesterone Receptor products
0.1 ml / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Progesterone Receptor 1154
Synonyms
PR, PGR, NR3C3, , Nuclear receptor subfamily 3 group C member 3
Product review
Please read our product review about Progesterone Receptor : Antibodies to Progesterone Receptor.
Product Description
Rabbit anti Progesterone Receptor 1154, Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Clone | 1154 |
| Catalog Number | SM7149P |
| Swiss Prot. No. | P06401 |
| Quantity | 0.1 ml |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7149P.pdf |
Datasheet Extract
| Background | There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
|
| Immunogen | Synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer190 of human progesterone receptor Swiss Num.: P06401 |
| Format | State: Liquid Ig fraction Purification: Protein G Chromatography BufferSystem: 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol |
| Applications | IHC (1:1000-1:2000).
Dot blots (1:1000 to 1:2000) and Western blots (1:1000 to 1:2000). Recommended Positive Control: T47D treated with sythetic progestin agonist R5020 (500 nM). |
| Specificity | This antibody is specific for Progesterone Receptor phospho Ser190. Species: Human. |
| Storage | For long-term storage -80°C is recommended, but short term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L., and Edwards, D.P.,Mol. Endocrinol., 14 (2000) 52 - 65.
2. Lin, V. C. L., Woon, C. T., Aw, S. E., Guo, C.,Endocrinology 144 (2003) 5650 -5657. |
| Pictures | Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific labeling of the ~90 kDa PR-A isorform and the ~120 kDa PR-B isoform of the Progesterone Receptor phosphorylated at Ser190.
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12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7149P Progesterone Receptor '.
