SM7118P CD261 / TNFRSF10A antibody
see related secondary antibodiessee all 50 CD261 / TNFRSF10A products
0.1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD261 / TNFRSF10A 32A1380
Synonyms
TNFRSF10A, APO2, DR4, TRAILR1, Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, CD261, , Tumor necrosis factor receptor superfamily member 10A
Product Description
Mouse anti CD261 / TNFRSF10A 32A1380, Presentation: Purified. Product is tested for Flow cytometry ( F ), Functional assay ( FN ), Western blot / Immunoblot ( WB )
Properties
| Applications | Flow cytometry ( F ), Functional assay ( FN ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 32A1380 |
| Catalog Number | SM7118P |
| Swiss Prot. No. | O00220 |
| Quantity | 0.1 mg |
| Price | US$ 425 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7118P.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | Cells undergo programmed cell death (Apoptosis) in response to various stimuli. Apoptosis is essential for morphogenesis, tissue homeostasis, and host defense (1) Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) (2). The ligand for this novel death receptor has been identified and termed TRAIL (3,4), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1) (5). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells. |
| Immunogen | Peptide corresponding to amino acid 1 to 20 of human DR4 mature protein Swiss Num.: O00220 |
| Format | State: Liquid Ig fraction Purification: Protein G chromatography BufferSystem: PBS containing 0.05 % BSA and 0.05 % sodium azide |
| Applications | Western Blot: 2 - 4 µg/ml.
Flow Cytometry: Please see reference: Kroll et al. 2005, for details. Neutralization: Please see reference Lu et al. 2002, for details. Recommended Positive Control: Daudi (a 57 kDa band should be observed). |
| Specificity | This antibody is specific for DR4 (NT). This antibody is routinely tested be western blot analysis. Species: Human. |
| Storage | Store the antibody (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
Avoid repeated freezing and thawing. Centrifuge vial before opening. Shelf life: One year from despatch. |
| References | Reference
1. Ellis, R.E., Yuan, J. and Horvitz, H.R. Annu. Rev. Cell Biol. 7: 663-698 (1991). 2. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. Science; 1997;276:111-113. 3. Wiley SR, et al. Immunity 1995;3:673-682. 4. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A. J. Biol. Chem. 1996;271:12687-90. 5. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T, Holler N, Tschopp J. Immunity 1997;7:831-836. Product Citations 1. Evidence that the human death receptor 4 is regulated by activator protein 1. Baoxiang Guan, Ping Yue, Reuben Lotan, Shi-Yong Sun. Oncogene 21, 3121-3129 (2002). 2. Differential Expression of Members of the Tumor Necrosis Factor a-related Apoptosis-inducing Ligand Pathway in Prostate Cancer Cells. Supriya Sridhar, Afshan A. Ali, Yayun Liang, M. Fathy El Etreby, Ronald W. Lewis, and M. Vijay Kumar. Cancer Res., 61: 7179-7183 (2001). 3. Interferon- sensitizes human hepatoma cells to TRAIL-induced apoptosis through DR5 upregulation and NF-kB inactivation. Masaya Shigeno, Kazuhiko Nakao, Tatsuki Ichikawa, Kasumi Suzuki, Atsushi Kawakami, Seigou Abiru, Seiji Miyazoe, Yuichi Nakagawa, Hiroki Ishikawa, Keisuke Hamasaki, Keisuke Nakata, Nobuko Ishii, Katsumi Eguchi. Oncogene 22, 1653 -1662 (2003). 4. Inflammatory cytokine regulation of TRAIL-mediated apoptosis in thyroid epithelial cells. J D Bretz, E Mezosi, T J Giordano, P G Gauger, N W Thompson, J R Baker, Jr. Cell Death and Differentiation 9, 274-286 (2002). 5. Differential Roles of RelA (p65) and c-Rel Subunits of Nuclear Factor B in Tumor Necrosis Factor-related Apoptosis-inducing Ligand Signaling. Xufeng Chen, Karthikeyan Kandasamy, and Rakesh K. Srivastava. Cancer Res.,63: 1059-1066 (2003). 6. Innate Direct Anticancer Effector Function of Human Immature Dendritic Cells. II. Role of TNF, Lymphotoxin-k, Fas Ligand, and TNF-Related Apoptosis-Inducing Ligand. Ganwei Lu, Bratislav M. Janjic, Jelena Janjic, Theresa L. Whiteside, Walter J. Storkus, and Nikola L. Vujanovic. J. Immunol., 168: 1831-1839 (2002). 7. Glioma Cells Deficient in Urokinase Plaminogen Activator Receptor Expression Are Susceptible to Tumor Necrosis Factora-related Apoptosis-inducing Ligand-induced Apoptosis. Bhavani Krishnamoorthy, Bryant Darnay, Bharat Aggarwal, Dzung H. Dinh, Gregory Kouraklis, William C. Olivero, Meena Gujrati, and Jasti S. Rao. Clin. Cancer Res., 7: 4195-4201 (2001). 8. p53 Upregulates Death Receptor 4 Expression through an Intronic p53 Binding Site. Xiangguo Liu, Ping Yue, Fadlo R. Khuri, and Shi-Yong Sun. Cancer Res., 64: 5078-5083 (2004). 9. Differential Regulation of the TRAIL Death Receptors DR4 and DR5 by the Signal Recognition Particle. Yan-Guo Ren, Klaus W. Wagner, Deborah A. Knee, Pedro Aza-Blanc, Marc Nasoff, and Quinn L. Deveraux. Mol. Biol. Cell, Sep 2004; 10.1091/mbc.E04-03-0184. 10. 4-Tertiary Butyl Phenol Exposure Sensitizes Human Melanocytes to Dendritic Cell-Mediated Killing: Relevance to Vitiligo. Tara M. Kroll, Hemamalini Bommiasamy, Raymond E. Boissy, Claudia Hernandez, Brian J. Nickoloff, Ruben Mestril and I. Caroline Le Poole. The Journal of Investigative Dermatology. 124 (4): 798-806 (2005). (Flow) |
| Pictures | Western blot analysis of 15 µg of total cell lysate from Daudi cells with anti-DR4 SM7118P at 2 µg/ml.
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14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7118P CD261 / TNFRSF10A'.
