SM7116PX Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
see related secondary antibodiessee all 83 Glyceraldehyde-3-Phosphate Dehydrogenase products
0.1 ml / US$ 495
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) 1D4
Synonyms
GAPDH, GAPD, EC 1.2.1.12, Glyceraldehyde-3-phosphate dehydrogenase, ,
Product review
Please read our product review about Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH): Antibodies to Loading Controls.
Product Description
Mouse anti Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) 1D4, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ) |
| Reactivity | Chicken ( Chk ), Human ( Hu ), Mamalia ( Mam ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 1D4 |
| Catalog Number | SM7116PX |
| Swiss Prot. No. | P04406 |
| Quantity | 0.1 ml |
| Price | US$ 495 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7116PX.pdf |
Datasheet Extract
| Background | Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH or GPDH) is one of the key enzymes involved in glycolysis; it catalyzes the reversible oxidative phosphorylation of glyceraldehydes-3-phosphate. The GAPDH gene is constitutively and stably expressed at high levels in almost all tissues and cells, and as such is considered to be a housekeeping gene. Housekeeping proteins like GAPDH are useful as loading controls for western blots and protein normalization. They are also useful for visualizing cells in microscopy assays. |
| Immunogen | Purified pig GAPDH enzyme Swiss Num.: P04406 |
| Format | State: Liquid antibody solution containing 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA |
| Applications | Western Blot: 1:1000-1:5000. On Western blots, GAPDH is detected as a band of approximately 36-40 kDa. GAPDH antibodies are widely used as loading controls for quantitative Western blotting, including normalizing Western blot results to GAPDH.
Immunofluorescence/Immunocytochemistry: 1:100-1:1000. Recommended Positive Control: HeLa, Jurkat, Daudi, 293, Rh30, A375m T98Gm HCT-116, Hep-2, ovary, stomach, lung, liver, heart. Most cell lines and tissues will be positive for GAPDH. |
| Specificity | Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH or GPDH) Species: Mouse, Human, Rat, Rabbit, Pig, Bovine, Chicken. |
| Storage | Store at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Dimethyl Sulfoxide Has an Impact on Epigenetic Profile in Mouse Embryoid Body. Misa Iwatani, Kohta Ikegami, Yuliya Kremenska, Naka Hattori, Satoshi Tanaka, Shintaro Yagi, and Kunio Shiota. Stem Cells, 24: 2549-2556 (2006).
2. Increased susceptibility to transcriptional changes with novel stressor in adrenal medulla of rats exposed to prolonged cold stress. Xiaoping Liua, Richard Kvetnanskyb, Lidia Serovaa, Anne Sollasa and Esther L. Sabbana. Molecular Brain Research, doi:10.1016/j.molbrainres.2005.07.019 On line publication. 3. Allelic Variation in the Hepatitis C Virus NS4B Protein Dramatically Influences RNA Replication. K J. Blight, J. Virology., DOI:10.1128/JVI.02481-06 (2007). |
| Pictures | Application of the GAPDH antibody as a protein loading control in Western blots. Total proteins from various human cell lysates were normalized using the GAPDH monoclonal antibody. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
