SM7115P I Kappa B alpha (IKBa) (cleaved) antibody
see related secondary antibodiessee all 19 I Kappa B alpha products
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti I Kappa B alpha (IKBa) (cleaved) 5D1623
Synonyms
IkappaB alpha,
Product Description
Mouse anti I Kappa B alpha (IKBa) (cleaved) 5D1623, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB ), Immunoprecipitation ( IP )
Properties
| Applications | Western blot / Immunoblot ( WB ), Immunoprecipitation ( IP ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 5D1623 |
| Catalog Number | SM7115P |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7115P.pdf |
Datasheet Extract
| Monoclonal Antibody to Cleaved alpha IκBα | |
| Concentration | 0.5 mg/ml |
| Background | NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis. |
| Immunogen | Synthetic peptide corresponding to the caspase-3 mediated cleavage site of IkBa |
| Format | State: Liquid Ig fration Purification: Protein G Chromatography BufferSystem: PBS containing 0.02 % sodium azide |
| Applications | Western blot: 1.0 µg/ml.
Immunoprecipitation: 1.0 - 2.0 µg/ml. Recommended positive control: anti-Fas treated Jurkat (30 min). |
| Specificity | This antibody is specific for Cleaved alpha IκBα. Species: Human. |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Verma, I.M., Stevenson, J.K., Schwarz, E.M., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735, 1995.
2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci USA 94: 11758 (1997). 3. DiDonato, J.A., Hayakawa, M., Rothwarf, D.M. Zandi, E., and Karin, M. Nature 388: 548- (1997). 4. Regnier, C.H., Ho, Y.S., Gao, X., Goeddel, D.V., Cao, Z., and Rothe, M. Cell 90, 373 (1997). 5. Mercurio, F., Zhu, H., Murray, B.W., Shevchenko, A., Bennett, B.L., Li, J., Young, D.B., Barbosa, M. Mann, M., Manning, A., Rao, A. Science 278: 860 (1997). 6. Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S.T., Weil, R., Agou, F., Kirk, H.E., Kay, R.J., and Israel, A. Cell 93: 1231-1240 (1998). 7. Barkett, M, Xue, D, Horvitz, H.R., and Gilmore, TD. J Biol Chem 272: 29419-29422 (1997). 8. Reuther, J.Y. and Baldwin, Jr., A.S. J Biol Chem 274: 20664-20670 (1999). |
| Pictures | Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. untreated cell lysate. Lane B. Jurkat cells treated with anti-Fas antibody for 30 mins.
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7115P I Kappa B alpha (IKBa) (cleaved)'.