SM7114P I Kappa B alpha (IKBa) pSer32/36 antibody
see related secondary antibodies
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti I Kappa B alpha (IKBa) pSer32/36 39A1413
Synonyms
IkappaB alpha,
Product Description
Mouse anti I Kappa B alpha (IKBa) pSer32/36 39A1413, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB ), Immunoprecipitation ( IP )
Properties
| Applications | Western blot / Immunoblot ( WB ), Immunoprecipitation ( IP ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 39A1413 |
| Catalog Number | SM7114P |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7114P.pdf |
Datasheet Extract
| Monoclonal Antibody to Phosphorylated IκBα (Ser32, Ser36) | |
| Concentration | 0.5 mg/ml |
| Background | NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (7,8). Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa. |
| Immunogen | Synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa. |
| Format | State: Liquid Ig fraction Purification: Protein G chromatography BufferSystem: PBS, 0.05 % BSA, 0.05 % sodium azide |
| Applications | Western blot analysis: 1.0 - 2.0 µg/ml.
Immunoprecipitation: 1.0 - 2.0 µg/ml. Recommended positive control: TNFa treated Jurkat. |
| Specificity | This antibody is specific for IkBa/IkBa (Ser32, Ser36). Species: Human, Mouse. |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Ghosh, S., May, M.J., and Kopp, E.B. Annu Rev. Immunol. 16: 225-260 (1998).
2. Baldwin, A.S. Annu. Rev. Immunol. 14: 694-681 (1996). 3. Regnier, C.H., Ho, Y.S., Gao, X.,. Cell 90, 373 (1997). 4. Mercurio, F., et Al, A. Science 278: 860 (1997). 5. Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S.T., Weil, R., Agou, F., Kirk, H.E., Kay, R.J., and Israel, A. Cell 93: 1231-1240 (1998). 6. Verma, I.M., Stevenson, J.K., Schwarz, E.M., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735, 1995. 7. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci USA 94: 11758 (1997). 8. DiDonato, J.A., Hayakawa, et Al,, M. Nature 388: 548- (1997). |
| Pictures | Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with SM7114P (lanes 1 & 2) or SM7041P (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that SM7114P detects specifically the phosphorylated form of IkBa.
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14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7114P I Kappa B alpha (IKBa) pSer32/36'.