SM7041P I Kappa B alpha (IKBa) antibody
see related secondary antibodiessee all 18 I Kappa B alpha products
0.1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti I Kappa B alpha (IKBa) 6A920
Synonyms
IkappaB alpha
Product Description
Mouse anti I Kappa B alpha (IKBa) 6A920, Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 6A920 |
| Catalog Number | SM7041P |
| Quantity | 0.1 mg |
| Price | US$ 405 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7041P.pdf |
Datasheet Extract
| Monoclonal Antibody to IκBα | |
| Concentration | 0.5 mg/ml |
| Background | NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa. |
| Immunogen | Recombinant protein corresponding to amino acid residues 32-291 of human IkBa |
| Format | State: Liquid Ig fraction Purification: Protein G chromatography BufferSystem: PBS containing 0.05 % BSA, 0.05 % sodium azide |
| Applications | Immunoprecipitation: 1 µg/ml.
Western blot (1.0 - 2.0 µg/ml). Positive control: Daudi, Jurkat, or NIH 3T3 (40 kDa band is observed). |
| Specificity | The antibody reacts with IkBa. Species: Human, Mouse. |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Verma, I.M., Genes. Dev. 9: 2723-2735, 1995.
2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci USA 94: 11758 (1997). 3. DiDonato, J.A., et al. Nature 388: 548- (1997). 4. Regnier, C.H., et al. Cell 90, 373 (1997). 5. Mercurio, F., et al. Science 278: 860 (1997). 6. Yamaoka, S., et al. Cell 93: 1231-1240 (1998). 7. Sanjo H, Kawai T, and Akira S. DRAKs, novel serine/threonine kinases related to death associated protein kinase that trigger apoptosis. J Biol Chem 273 (44): 29066-29071, 1998. 8. Matsumoto M, Takeda K, Sanjo H, and Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 18 (3): 1642-1651, 1998. |
| Pictures | Western blot analysis of IkBa using SM7041P at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7041P I Kappa B alpha (IKBa)'.
