SM7029P DNA Methyltransferase 3b (Dnmt3b) antibody
see related secondary antibodiessee all 13 DNA Methyltransferase 3b products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti DNA Methyltransferase 3b (Dnmt3b) 52A1018
Synonyms
DNMT3B ICF M.HsaIIIB
Product review
Please read our product review about DNA Methyltransferase 3b (Dnmt3b): Antibodies to DNA methyltransferases (Dnmt Family).
Product Description
Mouse anti DNA Methyltransferase 3b (Dnmt3b) 52A1018, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ), Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 52A1018 |
| Catalog Number | SM7029P |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7029P.pdf |
Datasheet Extract
| Monoclonal Antibody to Dnmt3b//(DNA methyltransferase 3b) | |
| Concentration | 0.5 mg/ml |
| Background | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
|
| Immunogen | Bacteria expressed HIS-tag recombinant mouse Dnmt3b |
| Applications | Western blot analysis (2-4 µg/ml).
Immunoprecipitation (1-2 µg/ml). Immunocytochemistry (1-2 µg/ml). Immunohistochemistry on paraffin sections (see references). ChIP (see references). |
| Specificity | This antibody is specific for Dnmt3b.
It does not crossreact with Dnmt3a. Species: Human, mouse. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription. Raffaella Santoro, Junwei Li, Ingrid Grummt. Nature Genetics 32, 393-396 (2002).
2. Double RNA Interference of DNMT3b and DNMT1 Enhances DNA Demethylation and Gene Reactivation. Yu-Wei Leu, Farahnaz Rahmatpanah, Huidong Shi, Susan H. Wei, Joseph C. Liu, Pearlly S. Yan and Tim Hui-Ming Huang. Cancer Research 63, 6110-6115, October 1, 2003. 3. Role of the DNA Methyltransferase Variant DNMT3b3 in DNA Methylation. Daniel J. Weisenberger, Mihaela Velicescu, Jonathan C. Cheng, Felicidad A. Gonzales, Gangning Liang, and Peter A. Jones. Mol. Cancer Res. 2: 62-72 (2004). 4. Induction of DNA methylation and gene silencing by short interfering RNAs in human cells. Hiroaki Kawasaki, Kazunari Taira. Nature 431: 211-217 (2004). 5. Reduced Genomic Cytosine Methylation and Defective Cellular Differentiation in Embryonic Stem Cells Lacking CpG Binding Protein. Diana L. Carlone, Jeong-Heon Lee, Suzanne R. L. Young, Erika Dobrota, Jill Sergesketter Butler, Joseph Ruiz, and David G. Skalnik. Molecular and Cellular Biology, 25 (12): 4881-4891 (2005). 6. Loss of Estrogen Receptor Signaling Triggers Epigenetic Silencing of Downstream Targets in Breast Cancer. Yu-Wei Leu, Pearlly S. Yan, Meiyun Fan, Victor X. Jin, Joseph C. Liu, Edward M. Curran, Wade V. Welshons, Susan H. Wei, Ramana V. Davuluri, Christoph Plass, Kenneth P. Nephew, and Tim H-M. Huang. Cancer Research 64, 8184-8192 (2004). (ChIP Assay) 7. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. Jian Feng, Hua Chang, En Li, Guoping Fan . Journal of Neuroscience Research. 79 (6): 734-746 (2005). 8. An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection. Torng-Sen Lin, Huei Lee, Ruei-A Chend, Ming-Lin Ho, Chia-Yu Lin, Ya-Hui Chen, Yi Yu Tsai, Ming-Chih Chou, and Ya-Wen Cheng. Cancer Letters, 226 (1): 77-84 (2005). (IHC on paraffin section). 9. Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells. Yi-Yong Qiu, Bernard L. Mirkin and Rama S. Dwivedi. Cancer Detection and Prevention. doi:10.1016/j.cdp.2005.05.004 online publication. (immunofluorescence) 10. Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development. Jessica L. MacDonald, Christopher S.Y. Gin and A. Jane Roskams. Developmental Biology, In Press, Corrected Proof, Available online 8 November 2005. (IHC on paraffin) 11. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. Axelle Loriot, Etienne De Plaen, Thierry Boon, and Charles De Smet. J. Biol. Chem., Feb 2006; 10.1074/jbc.M510469200. 12. Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome. Yoshihide Ueda, Masaki Okano, Christine Williams, Taiping Chen, Katia Georgopoulos, and En Li. Development, 133: 1183-1192 (2006). 13. Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L Zheng-Hua Xie, Yan-Nv Huang, Zhao-Xia Chen, Arthur D. Riggs, Jian-Ping Ding, Humaira Gowher, Albert Jeltsch, Hiroyuki Sasaki, Kenichiro Hata, and Guo-Liang Xu Hum. Mol. Genet., 15: 1375-1385 (2006). 14. Suppression of Intestinal Neoplasia by Deletion of Dnmt3b. Haijiang Lin, Yasuhiro Yamada, Suzanne Nguyen, Heinz Linhart, Laurie Jackson-Grusby, Alexander Meissner, Konstantinos Meletis, Grace Lo, and Rudolf Jaenisch. Mol. Cell. Biol., 26: 2976-2983 (2006). 15. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Sophie La Salle and Jacquetta M. Trasler. Developmental Biology, In Press, Accepted Manuscript, Available online 21 April 2006 (WB) 16. Inhibition of IFN-ã transcription by site-specific methylation during T helper cell development. Brendan Jones, Jianzhu Chen. The EMBO Journal 25, 2443-2452 (2006). (ChIP Assay) 17. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Akiko Tsumura, Tomohiro Hayakawa, Yuichi Kumaki, Shin-ichiro Takebayashi, Morito Sakaue, Chisa Matsuoka, Kunitada Shimotohno, Fuyuki Ishikawa, En Li, et al. Genes to Cells, 11 (7): 805-814 (2006). 18. Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation. Keisuke Nimura, Chisaki Ishida, Hiroshi Koriyama, Kenichiro Hata, Shinya Yamanaka, En Li, Kiyoe Ura, and Yasufumi Kaneda. Genes Cells, 11: 1225-1237 (2006). |
| Pictures | Left figure: Western blot analysis for Dnmt3b using SM7029P at 2 ug/ml against 10 µg of cell lysates of 293 transfected with mouse Dnmt3a (lane 1) and Dnmt3b (lane 2). A protein band of approximate mol. wt. of 110 kDa is only detected in 293 cells transfected with mouse Dnmt3b cDNA.
Right figure: Western blot analysis of Dnmt3b in NIH 3T3 cell lysate using SM7029P at 4 ug/ml. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'SM7029P DNA Methyltransferase 3b (Dnmt3b)'.
