SM7028P Dnmt3a antibody
see related secondary antibodiessee all 23 Dnmt3a products
0.1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Dnmt3a
Synonyms
DNMT3A2, M.HsaIIIA, , DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA
Product review
Please read our product review about Dnmt3a : Antibodies to DNA methyltransferases (Dnmt Family).
Product Description
Mouse anti Dnmt3a , Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ), Paraffin Sections ( P ), ChIP assay ( ChIP )
Properties
| Applications | Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ), Paraffin Sections ( P ), ChIP assay ( ChIP ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Catalog Number | SM7028P |
| Swiss Prot. No. | Q9Y6K1 |
| Quantity | 0.1 mg |
| Price | US$ 405 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7028P.pdf |
Datasheet Extract
| Monoclonal Antibody to//DNA methyltransferase 3a (DNMT3a) | |
| Concentration | 0.5 mg/ml |
| Background | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. |
| Immunogen | Bacteria expressed HIS-tag recombinant DNMT3a. The epitope was found to lie near the
C-terminus (aa705-908). Swiss Num.: Q9Y6K1 |
| Format | State: Liquid Ig fraction Purification: Protein G affinity chromatography BufferSystem: PBS containing 0.05% BSA and 0.05% sodium azide |
| Applications | Western blot analysis: 2 µg/ml, positive control: transfected cell lysate.
IF/ICC: 5 µg/ml, positive control: HeLa cells IHC (paraffin): see Yoo, et al. (2003) ChIP: see product citation (4) |
| Specificity | This antibody reacts with DNMT3a. Species: Human, mouse. |
| Storage | Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Xie S, Wang Z, Okano M, Nogami M, Li Y, He WW, Okumura K, and Li E. Gene 236: 87-95 (1999).
2. Okano M, Bell DW, Haber DA, and Li E. Cell 99: 247-257 (1999). 3. Reik W, Kelsey G, and Walter J. Nat. Genet 23: 380-382 (1999). 4. Hsieh CL. Mol Cell Biol 19: 8211-8218 (1999). 5. Hansen RS, Wijmenga C, Juo P., Stanek AM, canfield TK, Weemaes CM, and Gartler SM. Proc. Natl. Acad. Sci USA 96: 14412-14417 (1999). Product Citations 1. A Novel Dnmt3a Isoform Produced from an Alternative Promoter Localizes to Euchromatin and Its Expression Correlates with Active de Novo Methylation. Taiping Chen,Yoshihide Ueda, Shaoping Xie, and En Li. J. Biol. Chem, 277: 38746-38754 (2002). 2. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Kathleen Dennis, Tao Fan, Theresa Geiman, Qingsheng Yan, and Kathrin Muegge. Genes & Dev.,15: 2940-2944 (2001). 3. Association of Lsh, a Regulator of DNA Methylation, with Pericentromeric Heterochromatin Is Dependent on Intact Heterochromatin. Qingsheng Yan, Edward Cho, Stephen Lockett, and Kathrin Muegge. Molecular and Cellular Biology, , 23 (23) 8416-8428 (2003). 4. Loss of Estrogen Receptor Signaling Triggers Epigenetic Silencing of Downstream Targets in Breast Cancer. Yu⿿Wei Leu, Pearlly S. Yan, Meiyun Fan, Victor X. Jin, Joseph C. Liu, Edward M. Curran, Wade V. Welshons, Susan H. Wei, Ramana V. Davuluri, Christoph Plass, Kenneth P. Nephew, and Tim H-M. Huang. Cancer Research 64, 8184-8192 (2004). (ChIP Assay) 5. De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine. Masahiro Oka, Amy M Meacham, Takashi Hamazaki, Nemanja Rodi, Lung-Ji Chang and Naohiro Terada. Oncogene 24, 3091-3099 (2005). 6. Myc represses transcription through recruitment of DNA methyltransferase corepressor. Carmen Brenner, Rachel Deplus, Celine Didelot, Axelle Loriot, Emmanuelle Vir, Charles De Smet, Arantxa Gutierrez, Davide Danovi, David Bernard, Thierry Boon, Pier Giuseppe Pelicci, Bruno Amati, Tony Kouzarides, Yvan de Launoit, Luciano Di Croce, Francois Fuks. The EMBO Journal 24, 336-346 (2005). 7. Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model. Joanne M. Stempak, Kyoung-Jin Sohn, En-Pei Chiang, Barry Shane, and Young-In Kim. Carcinogenesis, 26: 981-990 (2005). 8. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. Jian Feng, Hua Chang, En Li, Guoping Fan. Journal of Neuroscience Research. 79 (6): 734-746 (2005). 9. Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells. Yi-Yong Qiu, Bernard L. Mirkin and Rama S. Dwivedi. Cancer Detection and Prevention. doi:10.1016/j.cdp.2005.05.004 online publication. (immunofluorescence). |
| Pictures | Western blot analysis for Dnmt3a using SM7028P at 2 ug/ml against 10 µg of 293 cell lysate transfected with either mouse Dnmt3a (lane A) or mouse Dnmt3b (lane B).
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7028P Dnmt3a '.