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Western blot analysis for Dnmt2 using SM7027P at 2 µg/ml dilution against 15 µg of transfected cell lysate

 

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SM7027P  Dnmt2 antibody

see related secondary antibodies
see all 9 Dnmt2 products
0.1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti Dnmt2

Synonyms

M.HsaIIP, PuMet, TRDMT1, , tRNA (cytosine-5-)-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP

Product review
Please read our product review about Dnmt2 : Antibodies to DNA methyltransferases (Dnmt Family).

Product Description

Mouse anti Dnmt2 , Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )

Properties

ApplicationsWestern blot / Immunoblot ( WB )
ReactivityMouse ( Ms ), Human ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
Catalog NumberSM7027P
Swiss Prot. No.O14717
Quantity0.1 mg
PriceUS$ 425
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM7027P.pdf

Datasheet Extract

Monoclonal Antibody to DNA methyltransferase 2 (DNMT2)
Concentration0.5 mg/ml
BackgroundMethylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
ImmunogenSynthetic peptide corresponding to mouse DNMT2
Swiss Num.: O14717
Format
State: Liquid Ig fraction
Purification: Protein G chromatography
BufferSystem: PBS containing 0.2% gelatin and 0.05% sodium azide
ApplicationsWestern blot analysis (1-3 µg/ml).
Positive control: transfected cell lysate (a 42 kDa band is oberseved).
SpecificityThis antibody is specific for Dnmt2.
Species: Human, Mouse.
StorageStore the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Yoder JA, Bestor TH. Hum Mol Genet 7(2):279-84 (1998).
2. Okano M, Xie S, Li E.: Nucleic Acids Res 26:2536-40 (1998).
PicturesWestern blot analysis for Dnmt2 using SM7027P at 2 µg/ml dilution against 15 µg of transfected cell lysate

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

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