SM7026P Dnmt1 antibody
see related secondary antibodiessee all 50 Dnmt1 products
0.1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Dnmt1 60B1220
Synonyms
M.Hsal, MCMT, AIM, CXXC9, DNMT, , DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, CXXC-type zinc finger protein 9
Product review
Please read our product review about Dnmt1 : Antibodies to DNA methyltransferases (Dnmt Family).
Product Description
Mouse anti Dnmt1 60B1220, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB ), Immunoprecipitation ( IP ), Paraffin Sections ( P )
Properties
| Applications | Western blot / Immunoblot ( WB ), Immunoprecipitation ( IP ), Paraffin Sections ( P ) |
| Reactivity | Mouse ( Ms ), Human ( Hu ), Zebrafish ( Zebrafish ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 60B1220 |
| Catalog Number | SM7026P |
| Swiss Prot. No. | P26358 |
| Quantity | 0.1 mg |
| Price | US$ 405 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7026P.pdf |
Datasheet Extract
| Monoclonal Antibody to Dnmt//(DNA methyltransferase) 1 | |
| Concentration | 0.5 mg/ml |
| Background | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. |
| Immunogen | Synthetic peptide corresponding to amino acids
637-651 human Dnmt1 (Genbank Accession No. NP_001370). Swiss Num.: P26358 AA Sequence: EKDDREDKENAFKR |
| Format | State: Liquid purified Ig fraction Purification: Protein G chromatography BufferSystem: PBS containing 0.05% BSA and 0.05% sodium azide as preservative |
| Applications | Western blot analysis (2-4 µg/ml, positive control: mouse ES cells).
Immunoprecipitation (1). ChIP (12). Immunohistochemistry on paraffin sections (1-2 µg/ml on human kidney cells). |
| Specificity | This antibody recognizes Dnmt1, a 1616 amino acid protein according to GenBank no. NP_001370. Species: Human, mouse, Zebrafish. |
| Storage | Store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Decrease of DNA methyltransferase 1 expression relative to cell proliferation in transitional cell carcinoma. Fumihiro Kimura, Hans-Helge Seifert, Andrea R. Florl, Simon Santourlidis, Christine Steinhoff , Sandra Swiatkowski , Csaba Mahotka , Claus-Dieter Gerharz , and Wolfgang A. Schultz. International J Cancer, 104 (5): 58-578 (2003). (Immunoprecipitation).
2. Components of a Pathway Maintaining Histone Modification and Heterochromatin Protein 1 Binding at the Pericentric Heterochromatin in Mammalian Cells. Huawei Xin, Ho-Guen Yoon, Prim B. Singh, Jiemin Wong, and Jun Qin. J. Biol. Chem., Vol. 279, 10: 9539-9546 (2004). 3. RNA Interference-Mediated Knockdown of DNA Methyltransferase 1 Leads to Promoter Demethylation and Gene Re-Expression in Human Lung and Breast Cancer Cells. Makoto Suzuki, Noriaki Sunaga, David S. Shames, Shinichi Toyooka, Adi F. Gazdar and John D. Minna. Cancer Research 64, 3137-3143 (2004). 4. Induction of DNA methylation and gene silencing by short interfering RNAs in human cells. Hiroaki Kawasaki, Kazunari Taira. Nature 431: 211-217 (2004). 5. Farnesyltransferase inhibitor (L-744,832) restores TGF-b type II receptor expression and enhances radiation sensitivity in K-ras mutant pancreatic cancer cell line MIA PaCa-2. Rachael A Alcock, Swatee Dey, Damodaran Chendil, Mohammed S Inayat, Mohammed Mohiuddin, George Hartman, Lee K Chatfield, Vincent S Gallicchio, Mansoor M Ahmed. Oncogene 21, 7883-7890 (2002). 6. The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription. Raffaella Santoro, Junwei Li, Ingrid Grummt. Nature Genetics 32, 393-396 (2002). 7. The Epstein-Barr virus oncogene product, latent membrane protein 1, induces the downregulation of E-cadherin gene expression via activation of DNA methyltransferases. Chi-Neu Tsai, Chia-Lung Tsai, Ka-Po Tse, Hwan-You Chang, and Yu-Sun Chang. PNAS, 99: 10084-10089 (2002). 8. Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter . Zhiyong Zhang, Chun Deng, Qianjin Lu and Bruce Richardson. Mechanisms of Ageing and Development 123: 1257-1268 (2002). 9. Double RNA Interference of DNMT3b and DNMT1 Enhances DNA Demethylation and Gene Reactivation. Yu-Wei Leu, Farahnaz Rahmatpanah, Huidong Shi, Susan H. Wei, Joseph C. Liu, Pearlly S. Yan and Tim Hui-Ming Huang. Cancer Research 63: 6110-6115 (2003). 10. Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns. Jeffrey M. Craig, Elizabeth Earle, Paul Canham, Lee H. Wong, Melissa Anderson, and K.H. Andy Choo. Hum. Mol. Genet., 12: 3109-3121 (2003). 11. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Kathleen Dennis, Tao Fan, Theresa Geiman, Qingsheng Yan, and Kathrin Muegge. Genes Dev. 15: 2940-2944 (2001). 12. Loss of Estrogen Receptor Signaling Triggers Epigenetic Silencing of Downstream Targets in Breast Cancer. Yu-Wei Leu, Pearlly S. Yan, Meiyun Fan, Victor X. Jin, Joseph C. Liu, Edward M. Curran, Wade V. Welshons, Susan H. Wei, Ramana V. Davuluri, Christoph Plass, Kenneth P. Nephew, and Tim H-M. Huang. Cancer Research 64, 8184-8192 (2004). (ChIP Assay) 13. Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. In Young Park, Bo Hwa Sohn, Jung Ha Choo, Cheol O. Joe, Je Kyung Seong, Young Ik Lee, Jae Hoon Chung. Journal of Cellular Biochemistry 94 (3) 585-596 (2005). 14. 5-Aza-Deoxycytidine Induces Selective Degradation of DNA Methyltransferase 1 by a Proteasomal Pathway That Requires the KEN Box, Bromo-Adjacent Homology Domain, and Nuclear Localization Signal. Kalpana Ghoshal, Jharna Datta, Sarmila Majumder, Shoumei Bai, Huban Kutay, Tasneem Motiwala, and Samson T. Jacob. Mol. Cell. Biol. 25: 4727-4741 (2005). (Immunocytochemitsry) 15. Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma. Bejai I.S. Srivastava and Maya D. Srivastava. Leukemia Research, 29 (7): 771-783 (2005). 16. Immunology of Cutaneous Vasculitis Associated with both Etanercept and Infliximab. Srivastava, M. D., Alexander, F. & Tuthill, R. J. Scandinavian Journal of Immunology, 61 (4): 329-336 (2005). 17. An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection. Torng-Sen Lin, Huei Lee, Ruei-A Chend, Ming-Lin Ho, Chia-Yu Lin, Ya-Hui Chen, Yi Yu Tsai, Ming-Chih Chou, and Ya-Wen Cheng. Cancer Letters, 226 (1): 77-84 (2005). (IHC on paraffin section). General References: 1. Bestor. T., et al.. J.Mol. Biol. 203: 971-983 (1988). 2. Yen RW, Vertino PM, Nelkin BD et al. Nucl. Acids Res. 20: 2287-2291 (1992). 3. Xie, S. et al. Gene 236: 87-95 (1999). 4. Okano M, Bell DW, Haber DA,a nd Li E. Cell 99: 247-257 (1999). 5. Reik W. et al. J. Nat. Genet 23: 380-382 (1999). 6. Robertson KD, et al. Nat Gen |
| Pictures | Western blot analysis for Dnmt1 using SM7026P at 2 ug/ml against 10 µg of cell lysates of mouse ES cell. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7026P Dnmt1 '.