SM7021P D4-GDI (GDP dissociation inhibitor) antibody
see related secondary antibodiessee all 23 D4-GDI products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti D4-GDI (GDP dissociation inhibitor) 10D774
Synonyms
D4GDI ARHGDIB Rho GDI beta
Product Description
Mouse anti D4-GDI (GDP dissociation inhibitor) 10D774, Presentation: Purified. Product is tested for Westernblot / Immunoblot ( WB )
Properties
| Applications | Westernblot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Canine ( Can ), Chicken ( Chk ), Human ( Hu ), Equine ( Eq ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG |
| Clone | 10D774 |
| Catalog Number | SM7021P |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7021P.pdf |
Datasheet Extract
| Monoclonal Antibody to Human D4-GDI | |
| Concentration | 0.5 mg/ml |
| Background | D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and
5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms. |
| Immunogen | Synthetic peptide corresponding to human D4-GDI AA Sequence: YVQHTYRTGVKVD Remarks: The peptide sequence used for immunogen is 100% conserved in human, dog, cow, chick, and horse. GenBank no. ref|NP_001166.3|. |
| Format | State: Liquid Ig fraction Purification: Protein G chromatography BufferSystem: PBS containing 0.02 % sodium azide and 0.02 % gelatin |
| Applications | Western blot analysis (1.0 - 2.0 µg/ml).
Positive control: Daudi or Jurkat. A 28 kDa band is observed. |
| Specificity | This antibody is specific for D4-GDI. Species: Chicken, Cow, Dog, Horse, Human. |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Na S, et al. J Biol Chem. 271:11209-13 (1996).
2. Krieser RJ, et al. Cell Death Differ 6:412-9 (1999). |
| Pictures | Western blot analysis of SM7021P in whole Jurkat cell lysate. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'SM7021P D4-GDI (GDP dissociation inhibitor)'.
