SM7004P Androgen Receptor / AR (pSer213/210) antibody
see related secondary antibodiessee all 36 Androgen Receptor / AR products
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Androgen Receptor / AR 156C135.2
Synonyms
Dihydrotestosterone Receptor, DHTR, NR3C4, , Nuclear receptor subfamily 3 group C member 4, testicular feminization, spinal and bulbar muscular atrophy, Kennedy disease, AIS, HUMARA, KD, SBMA, SMAX1, TFM
Product review
Please read our product review about Androgen Receptor / AR : Antibodies to Androgen Receptor.
Product Description
Mouse anti Androgen Receptor / AR 156C135.2, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG |
| Clone | 156C135.2 |
| Catalog Number | SM7004P |
| Swiss Prot. No. | P10275 |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7004P.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790 (Lin et al. 2003). The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis. |
| Immunogen | This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248.
(Note: S* refers to phosphorylated serine in the peptide sequence). Swiss Num.: P10275 |
| Format | State: Liquid purified IgG Purification: Protein G Chromatography BufferSystem: PBS, pH 7.2, containing 0.05% BSA and 0.05% sodium azide |
| Applications | Western blotting: 1-4 µg/ml. Stains a band of 110 kD; IGF-1 stimulated LNCaP (prostate cancer) cells can be used as positive control. |
| Specificity | Reacts highly specific with human phospho-Androgenreceptor. Predicted reactivity from sequence homology: Mouse, Rat, Monkey, Chimpanzee, Pig, Dog. |
| Add. Information | In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details.
The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen. |
| Storage | Store the antibody at 4-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
|
| References | 1. Suppression Versus Induction of Androgen Receptor Functions by the Phosphatidylinositol 3-Kinase/Akt Pathway in Prostate Cancer LNCaP Cells with Different Passage Numbers. Lin, H-K., Y-C Hu, L. Yang, S. Altuwaijri. 2003. J. Biol. Chem. 278: 50902-50907.
2. A neuroactive steroid 5a-androstane-3a,17Ã?-diol regulates androgen receptor level in astrocytes. Olga A. Agapova, Paula E. Malone, M. Rosario Hernandez. Journal of Neurochemistry, 98 (2): 355-363 (2006 ). 3. Observer variation in immunohistochemical analysis of protein expression, time for a change? Kirkegaard T, Edwards J, Tovey S, McGlynn L M, Krishna S N, Mukherjee R, Tam L, Munro A F, Dunne B & Bartlett J M S. Histopathology 48, 787â??794 (2006). 4. Down-regulation of Androgen Receptor by 3,3'-Diindolylmethane Contributes to Inhibition of Cell Proliferation and Induction of Apoptosis in Both Hormone-Sensitive LNCaP and Insensitive C4-2B Prostate Cancer Cells. Mohammad M.R. Bhuiyan, Yiwei Li, Sanjeev Banerjee, Fakhara Ahmed, Zhiwei Wang, Shadan Ali, and Fazlul H. Sarkar. Cancer Res., 66: 10064-10072 (2006). 5. Akt blocks ligand binding and protects against expanded polyglutamine androgen receptor toxicityIsabella Palazzolo, Barrington G. Burnett, Jessica E. Young, Phebe L. Brenne, Albert R. La Spada, Kenneth H. Fischbeck, Brian W. Howell, and Maria Pennuto. Hum. Mol. Genet., 16: 1593-1603 (2007). 6. Expression and degradation of androgen receptor: mechanism and clinical implication. Dong, KL and C. Chang. J Clinical Endocrinology and Metabolism. 88: 4043-4054 (2003). |
| Pictures | LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h. (See Lin, H-K., Y-C Hu, L. Yang, S. Altuwaijri. 2003 Fig. 2)
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14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7004P Androgen Receptor / AR '.
