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<b>Western blot analysis</b> Human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL4 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system * Lane 1 : Extracellular domain of KIR2DL1(23-223aa) Lane 2 : Extracellular domain of KIR2DL3(23-223aa) Lane 3 : Extracellular domain of KIR2DL4(44-202aa)

 

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SM6021  CD158 / KIR2D (L1, L3, L4, S4) antibody

see related secondary antibodies
0.1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD158 / KIR2D (L1, L3, L4, S4) 2H6

Synonyms

NKAT-1, NKAT1, NK Cell Marker, , Killer cell immunoglobulin-like receptor 2DL1, p58 NK receptor, NK Cell Marker

Product review
Please read our product review about CD158 / KIR2D (L1, L3, L4, S4): NK-Cell Markers.

Product Description

Mouse anti CD158 / KIR2D (L1, L3, L4, S4) 2H6, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )

Properties

ApplicationsEnzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG2b
Clone2H6
Catalog NumberSM6021
Swiss Prot. No.P43626
Quantity0.1 mg
PriceUS$ 425
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM6021.pdf

Datasheet Extract

Concentration1 mg/ml
BackgroundThe KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.
ImmunogenRecombinant human KIR2DL4 purified from E. coli
Swiss Num.: P43626
Format
State: Liquid Ig fraction
Purification: Protein A affinity chromatography
BufferSystem: Phosphate-Buffered Saline (pH 7.4) with 0.02 % thimerosal
ApplicationsELISA:
KIR2DL1: ++
KIR2DL3: +
KIR2DL4: +++
KIR2DS4: ++
Western blot (1:500 - 1:2,000):
KIR2DL1: +++
KIR2DL3: +++
KIR2DL4: ++
KIR2DS4: ++
SpecificityThis antibody recognises KIR2DL1, KIR2DL3, KIR2DL4 and KIR2DS4, members of killer cell immunoglobulin-like receptor family.
Species: Human.
StorageStore the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Shin JS, Shin EC, Kim J, Choi IH, Park JH, Kim SJ. 1999. Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors. Hybridoma 18:521-527.
2. Chwae YJ, Chang MJ, Park SM, Yoon H, Park HJ, Kim SJ, Kim J. 2002 Molecular mechanism of the activation-induced cell death inhibition mediated by a p70 inhibitory killer cell Ig-like receptor in Jurkat T cells. J Immunology 169:3726-3735.
PicturesWestern blot analysis Human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL4 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system * Lane 1 : Extracellular domain of KIR2DL1(23-223aa) Lane 2 : Extracellular domain of KIR2DL3(23-223aa) Lane 3 : Extracellular domain of KIR2DL4(44-202aa)

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

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