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<b>Western blot analysis</b> Human recombinant proteins, extracellular domain of NKp30(50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp30 (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates human recombinant protein NKp30 (residues 19~138)

 

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SM6020  CD337 (NKp30) antibody

see related secondary antibodies
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD337 (NKp30) y4E6

Synonyms

NK Cell Marker

Product Description

Mouse anti CD337 (NKp30) y4E6, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )

Properties

ApplicationsEnzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
Cloney4E6
Catalog NumberSM6020
Quantity0.1 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM6020.pdf

Datasheet Extract

Monoclonal Antibody to Human NKp30
Concentration1.0 mg/ml
BackgroundNatural cytotoxicity triggering receptor 3 (termed NKp30or NCR3) is a member of the Ig superfamily that may cooperate with NKp46 and NKp44 in the induction of cytotoxicity against a variety of target cells and is selectively expressed by all NK cells, both freshly isolated and cultured in IL-2, thus representing an optimal marker for NK cell identification. Similar to NKp46 and CD16, NKp30 associated with CD3ζ chains that become tyrosine phosphorylated following cell treatment with pervanadate. Its extracelluar portion is characterized by a single domain of the V-type and by a region rich in hydrophobic amino acids, potentially involved in protein/protein interactions that connects the Ig V-like domain with the transmembrane portion.
ImmunogenRecombinant extracellular domain (19-138aa) of human NKp30 (NCR3) purified from E. coli
Format
State: Liquid Ig fraction
Purification: Protein G affinity chromatography
BufferSystem: Phosphate-Buffered Saline (pH 7.4) with 0.02 % thimerosal
ApplicationsELISA.
Western blot.
SpecificityThis antibody is specific for NKp30 and does not cross react with NKp46 and NKp44.
Species: Human.
References1. Alessandro Moretta, Cristina Bottino, Massimo Vitale, Daniela Pende, Claudia Cantoni, Maria Cristina Mingari, Roberto Biassoni, Lorenzo Moretta. Activating receptors and coreceptors involved in human natural killer cell-mediated cytolysis. 2001 Annu.Rev.Immunol. 19:197-223.
2. Daniela Pende, Silvia Parolini, Anna Pessino, Simona Sivori, Raffaella Augugliaro, Luigia Morelli, Emanuela marcenaro, Laura Accame, Angela Malaspina, Roberto Biassoni, Cristina Bottino and Alessandro Moretta. Identification and molecular characterization of NKp30, a Novel triggering receptor involved in natural cytoxicity mediated by human natural killer cells. 1999 J Exp.Med. 15:1505-1516.
PicturesWestern blot analysis Human recombinant proteins, extracellular domain of NKp30(50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp30 (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates human recombinant protein NKp30 (residues 19~138)

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / 180,00 €
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / 160,00 €
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / 160,00 €
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / 170,00 €
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / 190,00 €
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / 200,00 €
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / 320,00 €
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / 320,00 €
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / 320,00 €
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / 320,00 €
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / 320,00 €
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / 330,00 €
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / 330,00 €
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / 330,00 €
   

Click here to see all secondary antibodies for 'SM6020 CD337 (NKp30)'.