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Profile of NK cells analyzed by flow cytometry

 

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SM6000  CD158b (KIR2DL3) antibody

see related secondary antibodies
see all 8 CD158b products
0.1 ml / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD158b (KIR2DL3) 190IIC311

Synonyms

NK Cell Marker Killer cell immunoglobulin-like receptor 2DL3, p58 NK receptor, p58.2 MHC class-I-specific NK receptor

Product review
Please read our product review about CD158b (KIR2DL3): NK-Cell Markers.

Product Description

Mouse anti CD158b (KIR2DL3) 190IIC311, Presentation: Purified. Product is tested for Flow cytometry ( F ), Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )

Properties

ApplicationsFlow cytometry ( F ), Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG2a
Clone190IIC311
Catalog NumberSM6000
Quantity0.1 ml
PriceUS$ 405
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM6000.pdf

Datasheet Extract

Monoclonal Antibody to Human KIR2DL3
Concentration1.0 mg/ml
BackgroundKIR2DL3 is a member of the killer cell immunoglobulin-like receptor(KIR) family. The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.
ImmunogenRecombinant human KIR2DL3
Format
State: Liquid Ig fraction
Purification: Protein-G affinity chromatography, 0.2 µm filtered
BufferSystem: Phosphate buffered saline, pH 7.4
ApplicationsFlow cytometry (5-10g/1x10e6).
ELISA (1:1,000 when tested against the immunizing protein).
Western blot (1 µg/ml).
SpecificityReacts with extracellular domain of the KIR2DL3.
Shows no reactivity with KIR2DL1 and KIR2DS4 in Western blot and ELISA.
Species: Human.
Add. InformationThis product is routinely tested on peripheral blood mononuclear cells(PBMCs) or natural killer(NK) cells. The histogram is an example Profile of NK cells analyzed by flow cytometry of the expected reactivity.
StorageStore the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Shin JS, Shin EC, Kim J, Choi IH, Park JH, Kim SJ. 1999. Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors. Hybridoma 18:521-527.
2. Chwae YJ, Chang MJ, Park SM, Yoon H, Park HJ, Kim SJ, Kim J. 2002 Molecular mechanism of the activation-induced cell death inhibition mediated by a p70 inhibitory killer cell Ig-like receptor in Jurkat T cells. J Immunology 169:3726-3735.
PicturesProfile of NK cells analyzed by flow cytometry

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SM6000 CD158b (KIR2DL3)'.