SM5166P Progesterone Receptor (pSer294) antibody
see related secondary antibodiessee all 77 Progesterone Receptor products
0.1 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Progesterone Receptor 608
Synonyms
PR, PGR, NR3C3, , Nuclear receptor subfamily 3 group C member 3
Product review
Please read our product review about Progesterone Receptor : Antibodies to Progesterone Receptor.
Product Description
Mouse anti Progesterone Receptor 608, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 608 |
| Catalog Number | SM5166P |
| Swiss Prot. No. | P06401 |
| Quantity | 0.1 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5166P.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | The progesterone receptor is a member of the Steroid Hormone Receptor Superfamily. It exists in two distinct isoforms in human cells. PR-B is the transcriptionally active form and is responsible for activating genes for the maintainence of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. Phospholabeling studies have shown that PR is phosphorylated in at least 9 different locations in response to progestins, which suggests that PR affects many different cellular pathways.
Ser 190 is one of four sites that demonstrate basal phosphorylation in the absence of ligand. The level of phosphorylation rapidly increases with addition of receptor agonists. This antibody recognizes the phosphorylated form of both PR-A and PR-B at Ser 190. |
| Immunogen | Synthetic Peptide corresponding to amino acid residues 288-300 from human progesterone receptor with serine 294 being phosphorylated: P(288) M A P G R S(p) P L A T T V(300). Swiss Num.: P06401 |
| Format | State: Liquid purified IgG fraction. BufferSystem: PBS containing 0.05% sodium azide as preservative and 1 mg/ml BSA as stabilizer. |
| Applications | ELISA.
Immunohistochemistry on paraffin embedded sections: 1/500. Immunoprecipitation. Western Blot: 10 µg/ml, detects ~81 and ~116 kDa proteins representing phospho-progesterone receptor (pSer294) A and B from T47D cell extract. |
| Specificity | SM5166P detects phospho-progesterone receptor (pSer294) from human samples. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Mol. Endo., 14(1):52-65, 2000.
2. Mol. Endo., 17(4):628-642, 2003. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5166P Progesterone Receptor '.
