SM5059 ABCC7 / CFTR antibody
see related secondary antibodiessee all 16 ABCC7 / CFTR products
0.1 ml / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti ABCC7 / CFTR CF3
Synonyms
Cystic fibrosis transmembrane conductance regulator, cAMP-dependent chloride channel
Product review
Please read our product review about ABCC7 / CFTR: Antibody Panel for the Detection of Cystic fibrosis transmembrane conductance regulator (CFTR).
Product Description
Mouse anti ABCC7 / CFTR CF3, Presentation: Ascites. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgM |
| Clone | CF3 |
| Catalog Number | SM5059 |
| Swiss Prot. No. | P13569 |
| Quantity | 0.1 ml |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5059.pdf |
Datasheet Extract
| Monoclonal Antibody to CFTR | |
| Background | Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport.
Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake. |
| Immunogen | Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117) corresponding to amino acid residues 103-117 found in the first extracellular loop of human and rabbit CFTR. Swiss Num.: P13569 Remarks: This sequence is highly conserved in mouse, sheep, cow, and Xenopus laevis. SM5059 immunizing peptide (SM5059CP) is available for use in Neutralization and Control experiments. |
| Format | State: Liquid diluted ascites containing sodium azide 0.05% as preservative. |
| Applications | Immunoprecipitation.
Immunofluorescence: 1/500, staining of CFTR results in cell surface staining in mouse epithelial cells, consistent with localization at the plasma membrane. Western Blot: 1/500, detects a single ~170 kDa protein representing CFTR in T84 whole cell extract. This antibody also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA. This antibody can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines. |
| Specificity | This antibody detects cystic fibrosis transmembrane conductance factor (CFTR) in human and mouse tissues. |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. FASEB J, Sep 2007; 21: 2939 - 2948.
2. J. Cell Science, 108: 2433-2444, 1995. 3. Nature, 393: 79-82, 1998. |
10 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1349B | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Biotin | 2 mg / 230,00 € | |||||
| R1349F | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | FITC | 2 mg / 210,00 € | |||||
| R1349T | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | TRITC | 2 mg / 210,00 € | |||||
| R1349TR | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Texas Red | 2 mg / 230,00 € | |||||
| R1349HRP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | HRP | 2 mg / 240,00 € | |||||
| R1349AP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | AP | 1 mg / 260,00 € | |||||
| SP1018F | Mouse IgM mu chain | ||||||
| Goat | FITC | Ms | 1 mg / 250,00 € | ||||
| SP1018R | Mouse IgM mu chain | ||||||
| Goat | PE | Ms | 0.1 mg / 350,00 € | ||||
| SP1018HRP | Mouse IgM mu chain | ||||||
| Goat | HRP | Ms | 1 mg / 260,00 € | ||||
| SP1018AP | Mouse IgM mu chain | ||||||
| Goat | AP | Ms | 0.8 mg / 300,00 € | ||||
Click here to see all secondary antibodies for 'SM5059 ABCC7 / CFTR'.
