SM5024 Estrogen Receptor alpha / ESR1 antibody
see related secondary antibodiessee all 119 Estrogen Receptor alpha / ESR1 products
50 µg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Estrogen Receptor alpha / ESR1 33
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Mouse anti Estrogen Receptor alpha / ESR1 33, Presentation: Ascites. Product is tested for Electrophoretic Mobility Shift Assay ( EMSA ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Electrophoretic Mobility Shift Assay ( EMSA ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 33 |
| Catalog Number | SM5024 |
| Swiss Prot. No. | P03372 |
| Quantity | 50 µg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5024.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins. |
| Immunogen | Synthetic peptide, corresponding to amino acid residues 247-261 of the ER alpha DNA binding domain Swiss Num.: P03372 AA Sequence: E(247) V G M K G G I R K D R R G(261) |
| Format | State: Liquid ascites diluted in PBS containing 0.09% sodium azide as preservative |
| Applications | Flow Cytometry.
Gel Shift. Immunocytochemistry (10 µg/ml). Immunohistochemistry (5 µg/ml): staining in mouse Leydig cells results in both nuclear and cytoplasmic staining). Immunoprecipitation. Western Blot (1 µg/ml): detects a ~67 kDa protein representing ER alpha from MCF-7 cell extract). |
| Specificity | This antibody detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha. SM5024 does not cross react with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor. Species: Bovine, human, non-human primate, mouse and rat tissues. |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | Biochem. Biophys. Res. Comm., 195(2): 730-736, 1993.
Biol Reprod, Vol 68: 1959-1967, Jun 2003 Blood, 87(7): 2683-2692, 1996. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5024 Estrogen Receptor alpha / ESR1 '.
