SM5022 TR beta-1 / THRB1 / NR1A2 antibody
see related secondary antibodiessee all 3 TR beta-1 / THRB1 / NR1A2 products
0.1 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti TR beta-1 / THRB1 / NR1A2 J52
Synonyms
Thyroid Hormone Receptor beta 1, THRB, THR1, ERBA2, , TR
Product review
Please read our product review about TR beta-1 / THRB1 / NR1A2 : Antibody Panel to Thyroid Hormone Receptor (TR).
Product Description
Mouse anti TR beta-1 / THRB1 / NR1A2 J52, Presentation: Ascites. Product is tested for Electrophoretic Mobility Shift Assay ( EMSA ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Electrophoretic Mobility Shift Assay ( EMSA ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Rat ( Rt ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | J52 |
| Catalog Number | SM5022 |
| Quantity | 0.1 ml |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5022.pdf |
Datasheet Extract
| Background | Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. |
| Immunogen | Purified human TR beta -1 overexpressed in E. coli.
|
| Format | State: Diluted ascites BufferSystem: PBS containing 0.05% sodium azide as preservative. |
| Applications | Immunocytochemistry (1/500, staining of TR beta-1 in intact GH3 cells results in nuclear staining; When levels of expression of the transfected receptor are high, cytoplasmic staining is seen along with nuclear staining). Gel Shift and Immunoprecipitation (reacts with the unliganded, liganded, and DNA binding forms of the receptor).
Western Blot (1/1000, detects a 52 and a 55 kDa protein representing recombinant TR expressed in E. coli; The two different sizes represent two different translational starting points). |
| Specificity | SM5022 detects thyroid hormone receptor (TR) beta-1 in human, rat and transformed bacterial cells. This antibody is specific for the TR beta-1 isoform. This antibody recognizes an epitope in the A/B domain of human TR beta-1, amino acid residues 1-101. |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | Endo., 128(5): 2601-2609, 1991.
Mol. Endo. 19(1): 102-112, 2005 MBC Vol 15: 1895-1903, 2004 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5022 TR beta-1 / THRB1 / NR1A2 '.
