SM5021 TR alpha-1/beta-1 antibody
see related secondary antibodies
0.1 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti TR alpha-1/beta-1 C3
Synonyms
THRA , ,
Product review
Please read our product review about TR alpha-1/beta-1: Antibody Panel to Thyroid Hormone Receptor (TR).
Product Description
Mouse anti TR alpha-1/beta-1 C3, Presentation: Ascites. Product is tested for Electrophoretic Mobility Shift Assay ( EMSA ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Electrophoretic Mobility Shift Assay ( EMSA ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | C3 |
| Catalog Number | SM5021 |
| Quantity | 0.1 ml |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5021.pdf |
Datasheet Extract
| Background | Thyroid hormone receptors (TR) are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. |
| Immunogen | Purified fragment of human TR beta-1 corresponding to amino acid residues 201-456. |
| Format | State: Liquid diluted ascites. BufferSystem: PBS containing 0.05% sodium azide as preservative. |
| Applications | Gel Shift.
Immunoprecipitation: SM5021 immunoprecipitates 52 kDa and 55 kDa proteins, representing TR beta-1 and a 48 kDa protein representing TR alpha-1 expressed in E. coli. Western Blot: 1/1000,detects a 55 and a 52 kDa protein representing recombinant human TR beta-1 expressed in E. coli. |
| Specificity | SM5021 detects thyroid hormone receptor (TR) from human tissues.
This antibody recognizes TR beta-1 and also cross-reacts with TR alpha-1. This antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414. This sequence is conserved in hTR alpha-1. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. J. Biol. Chem., Nov 2000; 275: 38032.
2. Endocrinology, Apr 2007; 148: 1764 - 1773 3. Mol. Cell. Biol., Nov 2000; 20: 8329 - 8342. 4. Mol Endocrinology 18(7), 1631-1642, Jul 2004 5. Molecular Endocrinology 10.1210/me.2003-0289 2005 6. JBC 2005, manuscript number M411514200. 7. Biochem. Biophys. Res. Comm., 195(1): 385-392, 1993. |
| Pictures | Figure 1. Western blot of human thyroid hormone receptor using SM5021. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5021 TR alpha-1/beta-1'.
