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SM3064P  Kappa Light Chain antibody

see related secondary antibodies
see all 135 Kappa Light Chain products
0.1 mg / US$ 285
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti Kappa Light Chain MEM-09

Product Description

Mouse anti Kappa Light Chain MEM-09, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsEnzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
CloneMEM-09
Catalog NumberSM3064P
Quantity0.1 mg
PriceUS$ 285
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM3064P.pdf

Datasheet Extract

Concentration1 mg/ml
BackgroundImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
ImmunogenCrude thymus membrane fraction.
Format
State: Liquid ascites
Purification: Protein A affinity chromatography, purity > 95 % (by SDS-PAGE)
BufferSystem: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
ApplicationsFlow Cytometry.
Immunoprecipitation.
Western Blotting.
Immunohistochemistry (paraffin sections).
Immunocytochemistry.
ELISA.
SpecificityThe antibody reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa-light chains. Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
Species: Human.
StorageStore the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
ReferencesHorejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha). 1986;32(1):12-25.

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

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