SM3001P Tubulin alpha antibody
see related secondary antibodiessee all 56 Tubulin alpha products
0.1 mg / US$ 295
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Tubulin alpha TU-16
Synonyms
Microtubule Marker, Loading Control, alpha-Tubulin, , ,
Product review
Please read our product review about Tubulin alpha: Antibodies to Loading Controls.
Product Description
Mouse anti Tubulin alpha TU-16, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | All Species ( All Species ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgM |
| Clone | TU-16 |
| Catalog Number | SM3001P |
| Quantity | 0.1 mg |
| Price | US$ 295 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM3001P.pdf |
Datasheet Extract
| Concentration | 1 mg/ml |
| Background | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain. |
| Immunogen | Porcine brain microtubule protein MTP-1 |
| Source | ascites. |
| Format | State: Liquid Ig fraction Purification: Precipitation methods; purity: > 95% (by SDS-PAGE) BufferSystem: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Applications | Immunoprecipitation.
Western Blotting (Reducing conditions): 1-2 mg/ml, 60 min. Positive control: HPB-ALL human peripheral blood leukemia cell line; sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail); Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Immunocytochemistry. ELISA. |
| Specificity | The antibody reacts with alpha-tubulin alltested species, under denaturing and non-denaturing conditions. (Recognized epitope is conserved within all species). |
| Storage | Store at 2-8°C. Do not freeze!
Shelf life: one year from despatch. |
| References | *Draberova E, Draber P.: Novel monoclonal antibodies TU-08 and TU-16 specific for tubulin subunits. Folia Biol (Praha). 1998;44(1):35-6.
*Drykova D, Cenklova V, Sulimenko V, Volc J, Dráber P, Binarova P.: Plant gamma-tubulin interacts with alphabeta-tubulin dimers and forms membrane-associated complexes. Plant Cell. 2003 Feb;15(2):465-80. *Qi H, Rath U, Wang D, Xu YZ, Ding Y, Zhang W, Blacketer MJ, Paddy MR, Girton J, Johansen J, Johansen K.M.: Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila. Mol Biol Cell. 2004 Nov;15(11):4854-65. *Ji Y, Rath U, Girton J, Johansen KM, Johansen J.: D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut. J Neurobiol. 2005 Aug;64(2):157-69. |
10 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1349B | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 355 | |||||
| R1349F | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 335 | |||||
| R1349T | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 335 | |||||
| R1349TR | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 355 | |||||
| R1349HRP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 365 | |||||
| R1349AP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | AP | 1 mg / US$ 385 | |||||
| SP1018F | Mouse IgM mu chain | ||||||
| Goat | FITC | Ms | 1 mg / US$ 375 | ||||
| SP1018R | Mouse IgM mu chain | ||||||
| Goat | PE | Ms | 0.1 mg / US$ 485 | ||||
| SP1018HRP | Mouse IgM mu chain | ||||||
| Goat | HRP | Ms | 1 mg / US$ 385 | ||||
| SP1018AP | Mouse IgM mu chain | ||||||
| Goat | AP | Ms | 0.8 mg / US$ 425 | ||||
Click here to see all secondary antibodies for 'SM3001P Tubulin alpha'.
