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Western blot analysis of human placenta extract probed with anti Human Cytochrome P450 aromatase (SM2222P) followed by F(ab')2 Rabbit anti Mouse IgG-HRP (SP1012HRP).

 

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SM2222PS  Aromatase (CYP19A1) antibody

see related secondary antibodies
see all 14 Aromatase products
0.2 ml / US$ 295
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti Aromatase (CYP19A1) H4

Synonyms

CYP19, ARO1, CYAR, p450AROM, CYPXIX, , Cytochrome P450 19A1, Estrogen synthetase

Product review
Please read our product review about Aromatase (CYP19A1): Antibodies to P450 Aromatase.

Product Description

Mouse anti Aromatase (CYP19A1) H4, Presentation: Supernatant. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsParaffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu ), Monkey ( Mky ), Rat ( Rt )
PresentationSupernatant
HostMouse
IsotypeIgG2a
CloneH4
Catalog NumberSM2222PS
Swiss Prot. No.P11511
Quantity0.2 ml
PriceUS$ 295
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM2222PS.pdf

Datasheet Extract

BackgroundP450 arom plays an important role in oestrogen biosynthesis and is highly conserved amongst mammals.
ImmunogenSynthetic peptide corresponding to amino acids 376 - 390 of human Aromatase

Swiss Num.: P11511
Format
State: Liquid 10x concentrated tissue culture supernatant
BufferSystem: 0.09% sodium azide as preservative
ApplicationsImmunohistochemistry on paraffin sections: does not require antigen retrieval using heat treatment. Recommended positive control tissue: human placenta.
Western Blot (1/250): detects a band of approximately 55 kD in human placental extracts.
P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots using this antibody (see ref. 1).
Description
AASequence: ALEDDVIDGYPVKKG








Format: This antibody is supplied as liquid, 10x concentrated tissue culture supernatant,
with 0.09% sodium azide as preservative.
SpecificitySM2222P recognises a conserved epitope within cytochrome P450 Aromatase (P450 arom).
Species: Human, Rat, Marmoset monkey.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Turner, K. J. et al. (2002) Development and validation of a new monoclonal antibody to mammalian aromtase.
J. Endocrinol. 172 (1): 21-30.
PicturesWestern blot analysis of human placenta extract probed with anti Human Cytochrome P450 aromatase (SM2222P) followed by F(ab')2 Rabbit anti Mouse IgG-HRP (SP1012HRP).

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SM2222PS Aromatase (CYP19A1)'.