SM2202P Tubulin alpha antibody
see related secondary antibodiessee all 56 Tubulin alpha products
0.5 mg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Tubulin alpha YL1/2
Synonyms
Microtubule Marker, Loading Control, alpha-Tubulin, , ,
Product review
Please read our product review about Tubulin alpha: Antibodies to Loading Controls.
Product Description
Rat anti Tubulin alpha YL1/2, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Radioimmunoassay ( R ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Radioimmunoassay ( R ), Western blot / Immunoblot ( WB ) |
| Reactivity | Avis ( Av ), Mamalia ( Mam ), Yeast ( Yeast ) |
| Presentation | Purified |
| Host | Rat |
| Isotype | IgG2a |
| Clone | YL1/2 |
| Catalog Number | SM2202P |
| Quantity | 0.5 mg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM2202P.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Immunogen | Yeast tubulin |
| Format | State: Liquid purfied Ig fraction. Purification: Protein G chromatography. BufferSystem: Phosphate buffered saline pH 7.4, containing 0.09% Sodium Azide as preservative. |
| Applications | Immunohistochemistry on frozen sections.
ELISA (1/100-1/1000; routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Clone YL1/2 has also been reported to work in western blotting (3). |
| Specificity | SM2202P recognises the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (5). Species: Yeast. Cross reacts with Mammals and Birds. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Kilmartin, J.V. et al. (1982). Rat monoclonal anti tubulin antibodies derived by using a new non secreting rat cell line. J. Cell. Biol. 93: 576-582.
2. Wehland, J. et al. (1983). A rat monoclonal antibody reacting specifically with the tyrosylated form of a tubulin. I. Biochemical characterization, effects of microtubule polymerization in vitro and microtubule polymerization and organization in vivo. J. Biol. 97: 1467-1475. 3. Wehland, J. et al. (1984). Amino acid sequence requirements in the epitope recognized by the alpha tubulin specific rat monoclonal antibody YL1/2. EMBO. J. 3: 1295-1300. 4. Burns, R. (1987). Tubulin's terminal tyrosine. Nature 327: 103-104. 5. Skinner, R.H. et al. (1991). Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating protein. J. Biol. Chem. 266: 14163-14166. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM2202P Tubulin alpha'.
