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Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN KIR (SM2036P).

 

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SM2036P  CD158 (KIR2DL, KIR2DS1, KIR2DS2, KIR2DS4) antibody

see related secondary antibodies
see all 7 CD158 products
0.2 mg / US$ 535
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD158 (KIR2DL, KIR2DS1, KIR2DS2, KIR2DS4) NKVFS1

Synonyms

NKAT-1 NKAT1 NK Cell Marker p58 NK receptor

Product review
Please read our product review about CD158 (KIR2DL, KIR2DS1, KIR2DS2, KIR2DS4): NK-Cell Markers.

Product Description

Mouse anti CD158 (KIR2DL, KIR2DS1, KIR2DS2, KIR2DS4) NKVFS1, Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )

Properties

ApplicationsEnzyme Immunoassay ( E ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationAff - Purified
HostMouse
IsotypeIgG1
CloneNKVFS1
Catalog NumberSM2036P
Quantity0.2 mg
PriceUS$ 535
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM2036P.pdf

Datasheet Extract

Monoclonal Antibody to Human KIR (CD158)
Alternate namesKILLER CELL IMMUNOGLOBIN-LIKE RECEPTORS
Concentration1.0 mg/ml
BackgroundKIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.
Format
State: Liquid purified IgG
Purification: Affinity chromatography on Protein G
BufferSystem: PBS, pH7.4 containing 0.09% Sodium Azide
ApplicationsFlow cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
ELISA.
Immunoprecipitation.
Western Blot.
SpecificityThis antibody recognises KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family, CD158a, CD158b and P50.3. Clone NKVFS1 specifically recognises the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). The clone is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms. We recommend the use of SM2036A for this purpose.
Species: Human.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Spaggiari, G. M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily. Blood. 99: 1706 - 1714.
2. Spaggiari, G. M. et al. (2002) Soluble HLA class I induces NK cell apoptosis upon the engagement of killeractivating HLA class I receptors through FasL-Fas interaction. Blood: 100: 4098 - 4107.
PicturesStaining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN KIR (SM2036P).

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SM2036P CD158 (KIR2DL, KIR2DS1, KIR2DS2, KIR2DS4)'.