SM1229P CD158b / KIR2DL3 antibody
see related secondary antibodiessee all 12 CD158b / KIR2DL3 products
0.2 mg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CD158b / KIR2DL3 GL183
Synonyms
NKAT-2, NKAT2, CD158B2, KIRCL23, NKAT2a NKAT2b, KIR-023GB, , , Killer cell immunoglobulin-like receptor 2DL3, p58 NK receptor, p58.2 MHC class-I-specific NK receptor
Product review
Please read our product review about CD158b / KIR2DL3 : NK-Cell Markers.
Product Description
Mouse anti CD158b / KIR2DL3 GL183, Presentation: Purified. Product is tested for Flow cytometry ( F ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Flow cytometry ( F ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | GL183 |
| Catalog Number | SM1229P |
| Quantity | 0.2 mg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM1229P.pdf |
Datasheet Extract
| Monoclonal Antibody to Human CD158b | |
| Alternate names | KIR2DL3 |
| Concentration | 1.0 mg/ml |
| Background | CD158b is a 58kD glycoprotein expressed by a subset of NK cells (also known as p58.2 antigen). The antigen is a members of the newly described natural killer cell receptor family. CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. This antibody can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3. |
| Immunogen | NK cell clone E57. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3UI myeloma cell line. Swiss Num.: P43628 |
| Format | State: Liquid purified IgG BufferSystem: PBS containing 0.09% Sodium Azide and 0.1% Bovine Serum Albumin |
| Applications | Flow cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunoprecipitation. |
| Specificity | This antibody recognises the CD158b cell surface antigen. This antibody also recognises a 50kD molecule, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail.
Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Moretta, A. et al. (1990) A novel surface antigen expressed by a subset of human CD3-CD16+ natural killer cells. J. Exp. Med. 171: 695 - 714.
2. Moretta, A. et al. (1993) p58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class-I protected cells in NK clones displaying different specificities. J. Exp. Med. 178: 597-604. 3. Ciccore, E. et al. (1992) Evidence of a natural killer (NK) cell repertoire for (allo) antigen recognition: definition of five distinct NK-determined allospecificities in humans. J. Exp. Med. 175: 709-718. 4. Moretta, A. et al. (1997) Major histocompatibility complex class I - specific receptors on human natural killer and T lymphocytes. Immunol Rev. 155: 105-117. 5. Moretta, A. et al. (1995) Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells. J. Exp. Med. 182: 875-884. 6. Biassoni, R. et al. (1996) The human leucocyte antigen (HLA)-c- specific "Activatory" or "Inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions. J. Exp. Med. 183: 645-650. 7. Long, E.O. et al. (1996) Inhibitory MHC Class I receptors on NK and T cells: a standard nomenclature. Immunol. Today 17: 100. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM1229P CD158b / KIR2DL3 '.