SM1229A CD158b (KIR2DL3) antibody
see related secondary antibodiessee all 8 CD158b products
0.2 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti CD158b (KIR2DL3) GL183
Synonyms
NK Cell Marker Killer cell immunoglobulin-like receptor 2DL3, p58 NK receptor, p58.2 MHC class-I-specific NK receptor
Product review
Please read our product review about CD158b (KIR2DL3): NK-Cell Markers.
Product Description
Mouse anti CD158b (KIR2DL3) GL183, Presentation: Azide Free. Product is tested for Flow cytometry ( F ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ), Functional assay ( FN )
Properties
| Applications | Flow cytometry ( F ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ), Functional assay ( FN ) |
| Reactivity | Human ( Hu ) |
| Presentation | Azide Free |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | GL183 |
| Catalog Number | SM1229A |
| Quantity | 0.2 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM1229A.pdf |
Datasheet Extract
| Monoclonal Antibody to Human CD158b (p58.2) | |
| Concentration | 0.2 mg/ml (prior to lyophilization) |
| Immunogen | NK cell clone E57 (CD2+, CD3-, CD7+, CD16+, CD56+) |
| Format | State: Lyophilized purified Ig fraction BufferSystem: PBS containing 1 mg/ml BSA as stabilizer containing no preservative |
| Applications | Flow cytometry: 2 µg / 5 x 10e5 cells (or 100 µl whole blood), per test.
Studies on the recognition mechanisms and function of NK cells and CTLs. Analysis of the NK cell repertoire. Immunoprecipitation of p50.2 and p58.2 molecules. |
| Specificity | GL183 monoclonal antibody immunoprecipitates the p58.2 molecule, a 58kDa type I transmembrane protein (1-3). It can also react with a lighter (p50.2, 50 kDa) molecule, highly homologous to p58.2 in the extracellular area, but exhibiting a shorter cytoplasmic tail.(4,5) These p58.2 and 50.2 molecules are characterized by two immunoglobulin-like extracellular domains. They are expressed by human NK cell subsets and by human T lymphocytes subsets, the latest being, in most instances, CD8+CD4-TCR alpha beta+.(6,7) p58.2 and p50.2 are members of the newly described natural killer cell receptor (NK-R) family (for reviews, see 8,9). The GL183 antibody can restore in vitro the lysis by human NK clones of otherwise lysis-protected targets carrying the Cw3 or related alleles. GL183 antibody has been assigned to the CD158b cluster of differentiation of the VIth International Workshop on Human Leucocyte Differentiation Antigens (HLDA) in Kobe, 1996. Species: Human. |
| Storage | Store lyophilized product at 2-8 °C. After reconstitution, aliquot and store at -20 °C. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch. |
| References | 1. Moretta, A., et al., (1990), A novel surface antigen expressed by a subset of human CD3-CD16+ natural killer cells, J. Exp Med., 171, 695-714.
2. Moretta, A., et al., (1990), Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition, J. Exp. Med., 172, 1589-1598. 3. Wagtmann, N., et al., (1995), Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains, Immunity, 2, 439-449. 4. Moretta, A., et al., (1995), Existence of both inhibotory (p58) and activatory (50) receptors for HLA-C molecules in human natural killer cells, J. Exp. Med., 182, 875-884. 5. Biassoni, R., et al., (1996), The human leukocyte antigen (HLA)-C-specific Activatory or Inhibitory natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions, J. Exp. Med., 183, 645-650. 6. Ferrini, S., et al., (1994), T cell clones expressing the natural killer cell-related p58 receptor molecule display heterogeneity in phenotypic properties and p58 function, Eur. J. Immunol., 24, 2294-2298. 7. Mingari, M.C., et al., (1996), Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations, Immunol., 93, 12433-12438. 8. Lopez-Botet, M., et al., (1996), NK-cell receptors and recognition of MHC class I molecules, Immunol. Today, 17, 5, 212-214. 9. Lanier, L.L. (1997), Natural killer cell receptors and MHC class I interactions, Current Opinion in Immunology, 9, 126-131. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM1229A CD158b (KIR2DL3)'.
