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Staining of human peripheral blood monocytes with Mouse anti Human CD33 using antibody SM1103P

 

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SM1103P  CD33 / SIGLEC3 antibody

see related secondary antibodies
see all 52 CD33 / SIGLEC3 products
0.2 mg / US$ 535
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD33 / SIGLEC3 WM53

Synonyms

CD33, SIGLEC3, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, Myeloid cell surface antigen CD33,

Product Description

Mouse anti CD33 / SIGLEC3 WM53, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )

Properties

ApplicationsFrozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
CloneWM53
Catalog NumberSM1103P
Swiss Prot. No.P20138
Quantity0.2 mg
PriceUS$ 535
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM1103P.pdf

Datasheet Extract

Concentration1.0 mg/ml
ImmunogenHuman AML cells
Swiss Num.: P20138
Format
State: Liquid IgG fraction
Purification: Protein G affinity chromatography
BufferSystem: PBS, pH 7.4, with 0.09% sodium azide as preservative
ApplicationsFlow cytometry (1/10 - 1/50): use 10 µl of the suggested working dilution to label 10e6 cells.
Immunoprecipitation.
Immunohistochemistry on frozen sections (1/50 - 1/100): Overnight incubation recommended. Positive control tissue: lymphatic tissue. (Not suitable on paraffin embedded sections!)
Western Blotting.
SpecificityRecognises the CD33 cell surface glycoprotein, a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.
Species: Human.
StorageStore the antibody undiluted at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Favaloro, E.J., Bradstock, K.F., Kabral, A., Grimsley, P. and Berndt, M.C. (1987). Characterisation of monoclonal antibodies to the human myeloid-specific antigen 'gp67' (CD-33). Disease Markers 5: 215-225.
2. Favaloro, E.J., Bradstock, K.F., Kabral, A., Grimsley, P., Zowtyj, H. and Zola, H. (1988). Further characterisation of myeloid antigens ('gp160,95', 'gp150' and 'gp67'): Investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD33. British Journal of Haematology 69: 163-171.
PicturesStaining of human peripheral blood monocytes with Mouse anti Human CD33 using antibody SM1103P

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SM1103P CD33 / SIGLEC3'.