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SM066F  Metallophilic Macrophages antibody

see related secondary antibodies
see all 3 Metallophilic Macrophages products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rat anti Metallophilic Macrophages MOMA-1

Synonyms

Mouse Macrophage Marker

Product Description

Rat anti Metallophilic Macrophages MOMA-1, Presentation: FITC. Product is tested for Flow cytometry ( F )

Properties

ApplicationsFlow cytometry ( F )
ReactivityMouse ( Ms )
PresentationFITC
HostRat
IsotypeIgG2a
CloneMOMA-1
Catalog NumberSM066F
Quantity0.1 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM066F.pdf

Datasheet Extract

Monoclonal Antibody to Murine CD169 // FITC
Alternate namesMetallophilic Macrophages
Concentration0.1 mg/ml
ImmunogenMouse macrophage cell lines.
Format
State: Liquid purified IgG
Purification: Affinity chromatography on Protein G
BufferSystem: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Label: Fluorescein Isothiocyanate Isomer 1 (FITC)
ApplicationsFlow Cytometry.
SpecificityThis antibody is specific for CD169, also known as sialoadhesin. This antibody reacts with a subpopulation of mature resident tissue macrophages. No reactivity is seen with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells or blood cells. Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localised at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localised in the sinusoids and medullary cords but not within follicular areas or paracortex. In Peyers patches they are localised in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1 although antigen expression is weaker than that seen in splenic macrophages. No MOMA-1 positive macrophages were found in the thymus. Reactivity was also negative in following organs tested so far (kidney, brain, skin). In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.
Species: Mouse.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
References1. Kraal, G. and Janse, M. (1986) Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody. Immunology 58: 665-669.
2. Oetke, C. et al. (2006) The antigen recognized by MOMA-I is sialoadhesin. Immunol Lett. 106: 96-98.
3. Kanayama, N. et al. (2005) Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone J. Immunol. 174:1438-45
4. Acevedo-Suarez, C.A. et al. (2005) Uncoupling of anergy from developmental arrest in anti-insulin B cells supports the development of autoimmune diabetes J Immunol. 174:827-833

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1371B Rat IgG (H&L)  
  Rabbit Biotin   2 mg / 170,00 €
   
R1371F Rat IgG (H&L)  
  Rabbit FITC   2 mg / 160,00 €
   
R1371T Rat IgG (H&L)  
  Rabbit TRITC   2 mg / 160,00 €
   
R1371TR Rat IgG (H&L)  
  Rabbit Texas Red   2 mg / 170,00 €
   
R1371HRP Rat IgG (H&L)  
  Rabbit HRP   2 mg / 190,00 €
   
R1371AP Rat IgG (H&L)  
  Rabbit AP   1 mg / 210,00 €
   
R1439C2 Rat IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / 320,00 €
   
R1439C3 Rat IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / 320,00 €
   
R1439C35 Rat IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / 320,00 €
   
R1439C5 Rat IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / 320,00 €
   
R1439C55 Rat IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / 320,00 €
   
R1413R Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh  
  Goat PE   1 ml / 330,00 €
   
R1428R Rat IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / 330,00 €
   
R1415R Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / 330,00 €
   

Click here to see all secondary antibodies for 'SM066F Metallophilic Macrophages'.