SM066B Metallophilic Macrophages antibody
see related secondary antibodiessee all 3 Metallophilic Macrophages products
0.2 mg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Metallophilic Macrophages MOMA-1
Synonyms
Mouse Macrophage Marker, ,
Product review
Please read our product review about Metallophilic Macrophages: Antibodies to Mouse Macrophages.
Product Description
Rat anti Metallophilic Macrophages MOMA-1, Presentation: Biotin. Product is tested for Frozen sections ( C ), Flow cytometry ( F )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Biotin |
| Host | Rat |
| Isotype | IgG2a |
| Clone | MOMA-1 |
| Catalog Number | SM066B |
| Quantity | 0.2 mg |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM066B.pdf |
Datasheet Extract
| Monoclonal Antibody to //Mouse Metallophilic Macrophages - Biotin | |
| Concentration | 0.4 mg/ml (after reconstitution) |
| Immunogen | Mouse lymph node tissue.
The antigen is found intracellularly and on cell surface on metallophilic macrophages. |
| Format | State: Lyophilized purified Ig fraction BufferSystem: PBS, pH 7.2, containing 5 mg/ml BSA as stabilizer and 0.05% Kathon as a preservative Label: Biotin Reconstitution: Restore by adding 0.5 ml distilled water. |
| Applications | Flow Cytometry.
Immunohistochemistry on frozen sections: approximate working dilution 0.5-1.0 μg/ml (1:400-1:800). Does not react on routinely processed paraffin sections. Suggested positive control: Mouse spleen. |
| Specificity | Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific
esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (product BM4011). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF). Antigen distribution Isolated cells: No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells. Tissue sections: Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine. Species: Mouse subpopulation of mature resident tissue macrophages. |
| Storage | Store the original vial at 2-8°C.
After reconstitution aliquot and store at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | Kraal, G., Janse, M.: Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody. Immunology 58, 665-669 (1986).
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM066B Metallophilic Macrophages'.