SM065 Macrophages/Monocytes antibody
see related secondary antibodiessee all 3 Macrophages/Monocytes products
0.2 mg / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Macrophages/Monocytes MOMA-2
Synonyms
Mouse Macrophage Marker
Product Description
Rat anti Macrophages/Monocytes MOMA-2, Presentation: Purified. Product is tested for Flow cytometry ( F ), Frozen sections ( C )
Properties
| Applications | Flow cytometry ( F ), Frozen sections ( C ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rat |
| Isotype | IgG2b |
| Clone | MOMA-2 |
| Catalog Number | SM065 |
| Quantity | 0.2 mg |
| Price | US$ 385 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM065.pdf |
Datasheet Extract
| Monoclonal Rat Antibody to//Mouse Macrophages/Monocytes | |
| Immunogen | Mouse lymph node stroma |
| Format | State: Lyophilized affinity purified Ig fraction BufferSystem: PBS, pH 7.2, containing 0.09% sodium azide as preservative and 5 mg/ml BSA as stabilizer. Reconstitution: Restore in 0.5 ml water (gives 0.4 mg/ml stock solution). |
| Applications | Flow cytometry (membrane permeabilisation is recommended).
Immunohistochemistry on fresh frozen sections (fixed with dry acetone at 4°C, use at 0.5 - 1 µg/ml (1:400 - 1:800). Suggested positive control: mouse spleen. Does not work on formalin-fixed, paraffin-embedded tissue sections. |
| Specificity | MOMA-2 is a useful marker for the broad detection of monocytes and macrophages in all mouse strains. In combination with the anti F4/80 marker BM8 (product BM4007) it allows a precise characterisation of tissue fixed macrophages in various organs. The antibody stains a mature macrophage subset, monocytes and a few precursors in bone marrow. Dendritic cells show low to intermediate expression. The staining shows close correlation with expression of acid phosphatase in tissue sections. MOMA-2 is predominantly expressed in the cytoplasm, but is also present on the cell surface.
MOMA-2 detects a (glyco-)protein of 140kD MW which is located within the cytoplasm and on the cell surface. The epitope has not been further characterized. Antigen distribution on isolated cells: In the cytospin preparation of thiogycollate stimulated peritoneal exudate cells MOMA-2 detects an antigen as distinct cytoplasmic spots. MOMA-2 detects monocytes of the peripheral blood and a subpopulation of bone marrow cells. Antigen distribution in tissue sections: MOMA-2 detects typical tissue macrophages as does the anti F4/80 specific clone BM8. However, different staining patterns are visible as shown below. The most predominant difference can be observed in T-cell areas and follicles of peripheral lymphoid organs where the anti F4/80 clone BM8 is negative. Species: Mouse. |
| Add. Information | The use of commercially available secondary antibodies that have been preadsorbed against mouse serum proteins may yield a weak staining. |
| Storage | Store the original vial at 2-8°C.
After reconstitution store (in aliquots) at -20°C. Avoid repeated freezing and thawing. Do not freeze working dilutions. Shelf life: one year from despatch. |
| References | KRAAL, G., REP, M., JANSE, M.: Macrophages in T and B Cell Compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2; An Immunohistochemical Study. Scand. J. Immunol.: 26, 653 - 661 (1987).
BREEL, M., MEBIUS, R.E., KRAAL,G.: Dendritic cells of the mouse recognized by two monoclonal antibodies. Eur. J. Immunol.: 17, 1555 - 1559 (1987). |
| Pictures | |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM065 Macrophages/Monocytes'.
